CAJ | 학술논문

建立了生咖啡中瘤曲霉毒素A的超高效液相色谱-串联质谱（UPLC—MS／MS）检测方法。样品用甲醇提取后．直接在BEHC18色谱柱上以乙腈-0．1％甲酸水为流动相分离，采用多离子监测模式（MRM）进行测定。赭曲霉毒素A的线性范围为5．0-200．0ng／mL，相关系数大于0．99，检出限为0．5μg／kg。4个水平的添加回收率在84．3％-109．2％．相对标准偏差小于7．35％。本方法灵敏度高、准确性好、成本低，适用于生咖啡中赭曲霉毒素A的检测。
건립료생가배중류곡매독소A적초고효액상색보-천련질보（UPLC—MS／MS）검측방법。양품용갑순제취후．직접재BEHC18색보주상이을정-0．1％갑산수위류동상분리，채용다리자감측모식（MRM）진행측정。자곡매독소A적선성범위위5．0-200．0ng／mL，상관계수대우0．99，검출한위0．5μg／kg。4개수평적첨가회수솔재84．3％-109．2％．상대표준편차소우7．35％。본방법령민도고、준학성호、성본저，괄용우생가배중자곡매독소A적검측。
An analytical method was developed for the determination of ochratoxin A in green coffee by using ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). OTA was extracted by methanol, separated through BEH C18 column using acetonitrile/water (containing 0.1% formic acid) as mobile phase, and then quantified with multiple reactions monitoring (MRM). Calibration curves exhibited good linearity over the concentration range from 5.0 to 200.0 ng/mL, with the correlation coefficients above 0.99. The limit of detection (LOD) for OTA was 0.5 izg/kg. Average recovery for OTA spiked at 4 levels ranged from 84.3% to 109.2%, with a relative standard deviations (RSD) less than 7.35%. The developed method was sensitive, accurate, low-cost, and can be applied for determination of OTA in green coffee.