CAJ | 학술논문

侵染后抗性胚状体诱导频率低是影响橡胶树遗传转化发展的主要原因．而橡胶树次生体胚发生体系具有体胚增殖效率及植株再生率高的优点。以3个综合性状优良的橡胶树无性系次生体细胞胚状体为受体，研究其对根癌农杆菌的耐侵染能力、GU$基因的瞬时表达情况、探讨不同无性系胚状体对农杆菌转化的反应。结果表明：3个无性系农杆菌污染情况无显著差异，长菌的外植体经无菌水和抗生索水洗涤3d后即可恢复生长；侵染后每30个胚褐化了1．2～5个胚．占侵染总胚数的4．00％16．67％，品种间褐化面积无显著差异；热研87—6—62的总蓝斑数显著高于其它2个无性系．平均每个胚染上14．29个蓝斑，热研7—33—97和PRl07稍差，但每个胚也能分别染上8．14和8．72个蓝斑。因此3个无性系均可以胚状体作为遗传转化的受体。此结果对利用胚状体为受体改良橡胶树优良无性系奠定良好基础。
침염후항성배상체유도빈솔저시영향상효수유전전화발전적주요원인．이상효수차생체배발생체계구유체배증식효솔급식주재생솔고적우점。이3개종합성상우량적상효수무성계차생체세포배상체위수체，연구기대근암농간균적내침염능력、GU$기인적순시표체정황、탐토불동무성계배상체대농간균전화적반응。결과표명：3개무성계농간균오염정황무현저차이，장균적외식체경무균수화항생색수세조3d후즉가회복생장；침염후매30개배갈화료1．2～5개배．점침염총배수적4．00％16．67％，품충간갈화면적무현저차이；열연87—6—62적총람반수현저고우기타2개무성계．평균매개배염상14．29개람반，열연7—33—97화PRl07초차，단매개배야능분별염상8．14화8．72개람반。인차3개무성계균가이배상체작위유전전화적수체。차결과대이용배상체위수체개량상효수우량무성계전정량호기출。
Low resistant embryo induction frequency after infection is the main reason affects the development of genetic transformation of Hevea, secondary somatic embryogenesis with high frequency in embryo multiplication and plant regeneration. In this study, secondary somatic embryos from three elite clones were used as receptor materials to study their reaction on Agrobacteriurn infection, including Agrobacterium contamination, browning and GUS transient expression. Results showed that there was no significant difference on Agrobacterium contamination and browning area between three clones, contaminated explants can restore after 3 days washing by sterile water followed by antibiotic water, and browning frequency was 4.00-16.67%, that is say 1.2-5 embryos browned after 30 embryos infected. The total number of CATAS 87-6-62 blue spots was significantly more than the other two clones, CATAS 7-33-97 and PR 107 slightly worse, but each embryo could also stained with 8.14 and 8.72 bh/e spots respectively. Therefore the three clones can use somatic embryos as a recipient for genetic transformation in Hevea. Results laid a good foundation for rubber tree elite clones improvement by using embryo as receptor.