农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2012年
12期
2560-2561,2666
,共3页
张贵贤%项方%王殿永%李国华
張貴賢%項方%王殿永%李國華
장귀현%항방%왕전영%리국화
水貂%绿脓杆菌%药敏试验
水貂%綠膿桿菌%藥敏試驗
수초%록농간균%약민시험
Mink%Pseudomonas aeruginosa%Drug sensitivity test
【目的]对水貂出血性肺炎进行诊断和防治。[方法]以秦皇岛市昌黎县荒田庄某养殖户饲养的水貂中发病水貂为试验材料,对其进行剖检,无菌取肝、肾、脾、肺等组织分别接种于普通营养琼脂和血液琼脂,分离、纯化细菌,并对获得的菌株进行动物接种试验和药敏试验。最后,提出相关治疗和预防措施。[结果]分离得到的细菌经革兰染色镜检为阴性;在血液琼脂上形成溶血环;该菌能发酵葡萄糖和木糖;不发酵蔗糖、乳糖、麦芽糖和七叶苷;过氧化氧酶和氧化酶试验阳性;尿素酶试验和硫化氢试验阴性.故确定该菌株为绿脓杆菌。药敏试验结果显示,该菌对环丙沙星等高敏,对头孢噻吩等耐药。[结论]该试验结果为水貂出仇性肺炎的诊治和预防奠定了基础。
【目的]對水貂齣血性肺炎進行診斷和防治。[方法]以秦皇島市昌黎縣荒田莊某養殖戶飼養的水貂中髮病水貂為試驗材料,對其進行剖檢,無菌取肝、腎、脾、肺等組織分彆接種于普通營養瓊脂和血液瓊脂,分離、純化細菌,併對穫得的菌株進行動物接種試驗和藥敏試驗。最後,提齣相關治療和預防措施。[結果]分離得到的細菌經革蘭染色鏡檢為陰性;在血液瓊脂上形成溶血環;該菌能髮酵葡萄糖和木糖;不髮酵蔗糖、乳糖、麥芽糖和七葉苷;過氧化氧酶和氧化酶試驗暘性;尿素酶試驗和硫化氫試驗陰性.故確定該菌株為綠膿桿菌。藥敏試驗結果顯示,該菌對環丙沙星等高敏,對頭孢噻吩等耐藥。[結論]該試驗結果為水貂齣仇性肺炎的診治和預防奠定瞭基礎。
【목적]대수초출혈성폐염진행진단화방치。[방법]이진황도시창려현황전장모양식호사양적수초중발병수초위시험재료,대기진행부검,무균취간、신、비、폐등조직분별접충우보통영양경지화혈액경지,분리、순화세균,병대획득적균주진행동물접충시험화약민시험。최후,제출상관치료화예방조시。[결과]분리득도적세균경혁란염색경검위음성;재혈액경지상형성용혈배;해균능발효포도당화목당;불발효자당、유당、맥아당화칠협감;과양화양매화양화매시험양성;뇨소매시험화류화경시험음성.고학정해균주위록농간균。약민시험결과현시,해균대배병사성등고민,대두포새분등내약。[결론]해시험결과위수초출구성폐염적진치화예방전정료기출。
[Objective] The paper was to diagnose and treat hemorrhagic pneumonia of mink. [Method] The diseased minks bred by one raiser in Huangtian Village of Changli County in Qinhuangdao City were used as experimental materials. It was necropsied, and its tissues such as liver, kidney, spleen and lung were taken under aseptic condition, then inoculated into general nutrition agar and blood agar. The bacteria were isolated and purified, and the strains obtained were carried out animal inoculation test and drug sensitivity test. Finally, the relevant treatment and preven- tive measures were put forward. [Conclusion] The bacteria isolated were detected to be negative by Gram staining; it formed hemolysis ring in blood agar; the bacteria could ferment glucose and xylose, which could not ferment sucrose, lactose, maltose and esculin; it was positive in catalase and oxidase test, and negative in urease test and hydrogen sulfide test; so the strain was indentified to be Pseudomonas aerugi- nosa. Drug sensitivity test showed that the bacteria were highly sensitive to ciprofloxacin, which had tolerance to cefoxitin. [Conclusion] The study laid foundation for diagnosis and treatment of hemorrhagic pneumonia of mink.