CAJ | 학술논문

目的探讨尖顶羊肚菌菌种在不同条件下的短期保藏对其菌丝生长的影响，以寻求适宜的菌种保藏条件：[方法]试验共设置9个处理．采用10℃／5℃（昼夜）和15℃／10℃两种变温条件、散射光和黑暗两种光照条件，分别保藏菌种30和60d的时间，以冰箱（4℃）保藏90天为对照。[结果]10℃／5℃保藏时．尖顶羊肚菌的菌丝生长优于15℃／10℃及对照保藏；散射光保藏菌落颜色和菌丝气生性及好于黑暗保减；在10℃／5℃温度下，保藏时间对菌丝生长影响不大，可以继续延长保藏时间，在15℃／10℃温度下，不宜超过60d的保藏时间。因此，较适宜的保藏条件是10℃／5℃、散射光、保藏30d，菌株表现为菌落边缘整齐，菌丝气生性中等，菌丝致密，菌落角变面积小，菌丝长速均匀稳定和菌丝干重较高。[结论]尖顶羊肚菌菌种在较低的常温、有散射光条件下保藏要比保藏在冰箱里效果佳。
목적 탐토첨정양두균균충재불동조건하적단기보장대기균사생장적영향，이심구괄의적균충보장조건：[방법]시험공설치9개처리．채용10℃／5℃（주야）화15℃／10℃량충변온조건、산사광화흑암량충광조조건，분별보장균충30화60d적시간，이빙상（4℃）보장90천위대조。[결과]10℃／5℃보장시．첨정양두균적균사생장우우15℃／10℃급대조보장；산사광보장균락안색화균사기생성급호우흑암보감；재10℃／5℃온도하，보장시간대균사생장영향불대，가이계속연장보장시간，재15℃／10℃온도하，불의초과60d적보장시간。인차，교괄의적보장조건시10℃／5℃、산사광、보장30d，균주표현위균락변연정제，균사기생성중등，균사치밀，균락각변면적소，균사장속균균은정화균사간중교고。[결론]첨정양두균균충재교저적상온、유산사광조건하보장요비보장재빙상리효과가。
[Objective] This study aimed to investigate the effect of different short-term preservation conditions on mycelial growth of Morchella conica, and search for opti- mum preservation conditions. [Method] M. conica strains in tubes were preserved at two temperature treatments 10 ℃/5 ℃ (day/night) and 15℃/10 ℃(day/night) in scat- tered light or dark for 30 or 60 d. The strain preserved at 4 ℃ dark for 90 d was the control (CK). So, a total of nine treatments were prepared in this study. [Result] Mycelial growth of M. conica preserved at 10 ℃/5 ℃ was better than that at 15 ℃/10 ℃ and control. The colony color and aerial hyphae of strains preserved in scattered light was also better than that in dark. Additionally, the preservation time showed no distinct effect on mycelial growth at 10 ℃/5 ℃. So the time could be lengthened. But the maximal preservation time was 60 d at 15 ℃/10 ℃. Therefore, preserved under the appropriate conditions of 10 ℃/5 ℃, scattered light for 30 d, the strain had the neat colony edge, moderate aerial mycelia, dense mycelia, lower sectoral variation, uniform mycelial growth rate and high dry weight of mycelia.