CAJ | 학술논문

[目的]探索延边黄牛与草原红牛钙蛋白酶1（CAPN1）基因4685位点对肉质嫩度的影响。[方法]采用PCR—RFLP技术对草原红牛、延边黄牛CAPN1基因第14内含子区4685位点进行基因多态性与肉质嫩度进行分析，验证该位点在吉林省地方品种牛中的作用。[结果]该位点与肉质嫩度相关检测指标（蒸煮损失、肌纤维直径、剪切力、滴水损失等）密切相关，均表现为T等位基因的存在能够显著提高个体的嫩度水平，但该位点与pH无关。4685位点与屠宰性状（净肉重、胴体重、净肉率等）无关，仅与眼肌面积存在一定的相关性。[结论]延边黄牛与草原红牛CAPN1基因4685位点与肉质嫩度存在密切相关。
[목적]탐색연변황우여초원홍우개단백매1（CAPN1）기인4685위점대육질눈도적영향。[방법]채용PCR—RFLP기술대초원홍우、연변황우CAPN1기인제14내함자구4685위점진행기인다태성여육질눈도진행분석，험증해위점재길림성지방품충우중적작용。[결과]해위점여육질눈도상관검측지표（증자손실、기섬유직경、전절력、적수손실등）밀절상관，균표현위T등위기인적존재능구현저제고개체적눈도수평，단해위점여pH무관。4685위점여도재성상（정육중、동체중、정육솔등）무관，부여안기면적존재일정적상관성。[결론]연변황우여초원홍우CAPN1기인4685위점여육질눈도존재밀절상관。
[Objective] The paper was to explore the impact of 4685 loci on CAPN1 gene of Yanbian yellow cattle and red steppe cattle on meat tenderness. [Method] The 4685 loci in 14th intron region of CAPN1 gene of Yanbian yellow cattle and red steppe cattle was conducted analysis of gene polymorphism and meat tenderness by PCR-RFLP technology, and the effect of the loci on Jilin local cattle breeds was verified. [Result] The loci had close relationship with related detection indices of meat tenderness (cooking loss, muscle fibre diameter, shear force and drip loss), and the existence of T allele could significantly increase the tenderness level of in- dividuals, but the loci was not associated to pH value. 4685 loci was not associated to carcass traits (carcass weight, net meat weight and carcass yield ratio), which only had certain correlation with eye muscle area. [Conclusion] The 4685 loci on CAPN1 gene of Yanbian yellow cattle and red steppe cattle had close association with meat tenderness.