CAJ | 학술논문

[目的]从免疫基因中挖掘调控羊毛性状的候选主效基因。[方法]利用芯片技术，检测敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达。[结果]差异倍数在2．0以上的免疫基因共46个，并对其中6个基因进行了实时定量PCR验证，其结果表明芯片数据和实时定量PCR验证结果相符率达66．67％，说明芯片数据是可靠的。[结论]免疫赦免机制可能参与羊毛的生长调控。
[목적]종면역기인중알굴조공양모성상적후선주효기인。[방법]이용심편기술，검측오한세모양체측부여복고구부피부면역기인적차이표체。[결과]차이배수재2．0이상적면역기인공46개，병대기중6개기인진행료실시정량PCR험증，기결과표명심편수거화실시정량PCR험증결과상부솔체66．67％，설명심편수거시가고적。[결론]면역사면궤제가능삼여양모적생장조공。
[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin (more wool growing) and groin skin (no wool growing) of Aohan fine wool sheep. [Results] 46 immune genes (fold change 〉2.0) were identified and classified, and then 6 of which were selected for QPCR confir- mation. The degree of consistency of the QPCR and microarray results was 66.67%, [Conclusion] Immune privilege may participate in wool growth regulation.