CAJ | 학술논문

目的：探讨土茯苓中药复方制剂和大蒜油的排铅效果，为铅中毒的防治提供一定的理论依据。方法：以40mg／L的醋酸铅溶液，按0．01mug体重剂量连续15d灌胃造模，造模成功后将48只雄性成年小鼠随机分成4组，即土茯苓中药复方制剂组、大蒜油组、乙二胺四乙酸组和中毒不治疗组，连续15d驱铅治疗。在造模前、第16天、第31天分别测定各组小鼠的体重及抽取小鼠尾部全血并用石墨炉原子吸收法直接测定小鼠的血铅含量，对实验结果进行统计学分析。结果：①血铅含量：统计学意义驱铅治疗后，土茯苓中药复方制剂组、大蒜油组、乙二胺四乙酸组分别与中毒不治疗组的血铅含量比较，差异均有统计学意义（P〈0．01）；土茯苓中药复方制剂组、大蒜油组、乙二胺四乙酸组的血铅含量两两比较，差异有统计学意义（P〈0．01）。驱铅治疗后各组的血铅含量为：土茯苓中药复方制剂组〈EDTA组〈大蒜油组〈中毒不治疗组。（参体重：土茯苓中药复方制剂组、大蒜油组分别与中毒不治疗组的体重比较，差异有统计学意义（P〈0．05）；土茯苓中药复方制剂组与大蒜油组的体重比较，差异有统计学意义（P〈0．05）。土茯苓中药复方制剂组的体重〉大蒜油组的体重。③存活率：土茯苓中药复方制剂组、大蒜油组分别与中毒不治疗组的存活率比较，差异有统计学意义（P〈0．01）；土茯苓中药复方制剂组与大蒜油组的存活率比较，差异无统计学意义（P〉O．05）。结论：土茯苓中药复方制剂、大蒜油对血铅均有促排作用；驱铅功效为：土茯苓中药复方制剂〉大蒜油。
목적：탐토토복령중약복방제제화대산유적배연효과，위연중독적방치제공일정적이론의거。방법：이40mg／L적작산연용액，안0．01mug체중제량련속15d관위조모，조모성공후장48지웅성성년소서수궤분성4조，즉토복령중약복방제제조、대산유조、을이알사을산조화중독불치료조，련속15d구연치료。재조모전、제16천、제31천분별측정각조소서적체중급추취소서미부전혈병용석묵로원자흡수법직접측정소서적혈연함량，대실험결과진행통계학분석。결과：①혈연함량：통계학의의구연치료후，토복령중약복방제제조、대산유조、을이알사을산조분별여중독불치료조적혈연함량비교，차이균유통계학의의（P〈0．01）；토복령중약복방제제조、대산유조、을이알사을산조적혈연함량량량비교，차이유통계학의의（P〈0．01）。구연치료후각조적혈연함량위：토복령중약복방제제조〈EDTA조〈대산유조〈중독불치료조。（삼체중：토복령중약복방제제조、대산유조분별여중독불치료조적체중비교，차이유통계학의의（P〈0．05）；토복령중약복방제제조여대산유조적체중비교，차이유통계학의의（P〈0．05）。토복령중약복방제제조적체중〉대산유조적체중。③존활솔：토복령중약복방제제조、대산유조분별여중독불치료조적존활솔비교，차이유통계학의의（P〈0．01）；토복령중약복방제제조여대산유조적존활솔비교，차이무통계학의의（P〉O．05）。결론：토복령중약복방제제、대산유대혈연균유촉배작용；구연공효위：토복령중약복방제제〉대산유。
Objective: To compare the lead excretion effect of smilax glabra and garlic oil, and provide the theoretical evidence to prevent lead poisoning. Methods: Lead poisoning model was constructed by lead acetate solution (40 mg/L) lavage at the standard of 0.01 mL/g weight for 15 days. After the lead exposure, 48 mice were randomly divided into 4 groups treated with smilax glabra, garlic oil, EDTA and distilled water respectively for 15 days. Mice weights and lead concentrations were recorded before the lead poisoning, the 16th day and the 31st day. Lead concentrations of tail vein blood were determined by the method of graphite furnace atomic absorption. Results: (~)With different treatment, there were significant differences on the lead content in the blood among smilax glabra group, EDTA group, garlic oil group and distilled water group (P〈0.01). After the therapy of the lead excreation, the blood lead content in smilax glabra group〈EDTA group〈garlic oil group〈 distilled water group. Compared to the distilled water group, there were both significant differences in weight of smilax glabra group and garlic oil group (P〈0.05); the weight of the smilax group was heavier than that of the garlic oil group (/9〈0.05). (~)Compared to the distilled water group, there were both significant differences in survival rate of smilax glabra group and garlic oil group (P〈0.05); there was no statistical difference on the survival rate of the smilax glabra group and garlic oil group (P〉0.05). Conclusion: Smilax glabra and garlic oil can promote the excretion of lead, our data showed better lead excretion effect of Smilax glabra compared to the garlic oil.