CAJ | 학술논문

目的：观察丝胶对2型糖尿病大鼠胰岛细胞神经肽Y（NPY）表达的影响。方法36只雄性 SD大鼠随机分为正常对照组、糖尿病模型组和丝胶治疗组，每组12只。链脲佐菌素腹腔注射建立2型糖尿病大鼠模型并给予丝胶（2.4 g/kg）灌胃。 SP免疫组织化学染色法观察大鼠胰岛细胞NPY的表达。结果 NPY蛋白阳性产物位于胰岛α细胞细胞质，呈棕黄色颗粒状。糖尿病模型组大鼠胰岛α细胞NPY蛋白的表达明显高于正常对照组大鼠（ P＜0.01）；丝胶治疗组大鼠胰岛α细胞NPY蛋白的表达明显低于糖尿病模型组大鼠（ P＜0.01）。结论丝胶可通过降低神经肽Y的表达保护糖尿病时胰岛细胞损伤。
목적：관찰사효대2형당뇨병대서이도세포신경태Y（NPY）표체적영향。방법36지웅성 SD대서수궤분위정상대조조、당뇨병모형조화사효치료조，매조12지。련뇨좌균소복강주사건립2형당뇨병대서모형병급여사효（2.4 g/kg）관위。 SP면역조직화학염색법관찰대서이도세포NPY적표체。결과 NPY단백양성산물위우이도α세포세포질，정종황색과립상。당뇨병모형조대서이도α세포NPY단백적표체명현고우정상대조조대서（ P＜0.01）；사효치료조대서이도α세포NPY단백적표체명현저우당뇨병모형조대서（ P＜0.01）。결론사효가통과강저신경태Y적표체보호당뇨병시이도세포손상。
Objective To observe the effects of sericine on neuropeptide Y ( NPY) expression in islet cells of type 2 diabetes melli-tus rats.Methods Methods 36 male SD rats were randomly divided into 3 groups with 12 rats in each group:normal control , diabetes mel-litus model and sericine treatment groups .Type 2 diabetes mellitus rat model was made by continuous intraperitoneal injection of streptozoto -cin, and then the rats were lavaged with sericine (2.4g/kg).SP immunohistochemical stain was used to observe NPY expression in islet cells.Results NPY positive immunoreactive products were located in cytoplasm of islet alpha cells .Compared with normal control rats , the NPY expression in alpha cells of rats in diabetes mellitus model group obviously increased (P<0.01).The NPY expression in alpha cells of rats in sericine treatment group was obviously lower than that of rats in diabetes mellitus model group (P<0.01).Conclusions Sericine can protect islet alpha cells by down regulating NPY expression during diabetes mellitus .