CAJ | 학술논문

目的：建立硫酸头孢噻利含量测定的反相高效液相色谱方法。方法采用HPLC-UV法测定，对乙酰氨基酚为内标，Kromasil C18柱（4.6 mm×250 mm，5μm）为分析柱，50 mmol?L-1磷酸二氢钠溶液-乙腈（90：10），流速为0.8 mL?min-1，检测波长为254 nm。结果头孢噻利在20～640μg?mL-1检测浓度范围内呈良好线性关系（r=0.9999），平均回收率为（99.19±0.64）%；日内差异RSD≤0.78%（n=5），日间差异RSD≤1.83%（n=5）。结论该方法操作简便、灵敏、准确，适用于头孢噻利的含量检测。
목적：건립류산두포새리함량측정적반상고효액상색보방법。방법채용HPLC-UV법측정，대을선안기분위내표，Kromasil C18주（4.6 mm×250 mm，5μm）위분석주，50 mmol?L-1린산이경납용액-을정（90：10），류속위0.8 mL?min-1，검측파장위254 nm。결과두포새리재20～640μg?mL-1검측농도범위내정량호선성관계（r=0.9999），평균회수솔위（99.19±0.64）%；일내차이RSD≤0.78%（n=5），일간차이RSD≤1.83%（n=5）。결론해방법조작간편、령민、준학，괄용우두포새리적함량검측。
Objective To establish a HPLC method for determination of the Cefoselis Sulfate. Methods The HPLC-UV method used Kromasil C18 (4.6 mm×250 mm, 5μm) column as an analytical column, a mobile phase consisted of 50 mmol?L-1 NaH2PO4-acetonitrile (90:10), at a lfow rate of 0.8 mL?min-1, and UV detection at 254 nm. The acetaminophen was used as internal standard. Results The assay was linear over the concentration range of 20~640μg?mL-1 (r=0.9999). The average recovery was (99.19±0.64)%. Intra-day and inter-day preci-sion showed coefifcients of variation within 0.78%(n=5) and 1.83%(n=5) over the three concentration levels. Conclusion The method is accurate, sensitive and simple, and can be used to detect the concentration of Cefoselis Sulfate.