中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
8期
1571-1573
,共3页
雷虹%尹海辉%章庆春%严中亚
雷虹%尹海輝%章慶春%嚴中亞
뢰홍%윤해휘%장경춘%엄중아
心脏移植%血管病变%热休克蛋白27%糖调节蛋白78
心髒移植%血管病變%熱休剋蛋白27%糖調節蛋白78
심장이식%혈관병변%열휴극단백27%당조절단백78
Cardiac allograft%Vasculopathy%Heat shock protein 27%Glucose regulated protein 78
目的 观察移植后心脏组织中热休克蛋白27(HSP27)和糖调节蛋白78(GRP78)的组织分布,探讨移植后心脏血管病变(CAV)的发病机制.方法 建立大鼠同基因(n=8)和异基因(n=8)心脏移植的Ono模型,采用免疫组织化学法[链霉菌抗生物素蛋白-过氧化物酶(SP)法]验证移植后心脏组织中HSP27和GRP78的组织分布.结果 同基因植组,HSP27和GRP78在心脏组织内均匀分布；异基因移植组,HSP27和GRP78在环血管周围心肌组织中高表达(2.24±0.36比1.04±0.10,P＜0.01；1.51±0.12比0.85±0.30,P＜0.05),而在增生的冠状动脉组织内几乎不表达(2.24±0.36比0.25±0.08,P＜0.01；1.51±0.12比0.16±0.10,P＜0.01).结论 HSP27和GRP78在异基因移植后心脏组织中分布不均,导致冠状动脉组织无力对抗免疫损伤,诱发血管平滑肌细胞过度增殖可能是CAV发生、发展的重要原因.
目的 觀察移植後心髒組織中熱休剋蛋白27(HSP27)和糖調節蛋白78(GRP78)的組織分佈,探討移植後心髒血管病變(CAV)的髮病機製.方法 建立大鼠同基因(n=8)和異基因(n=8)心髒移植的Ono模型,採用免疫組織化學法[鏈黴菌抗生物素蛋白-過氧化物酶(SP)法]驗證移植後心髒組織中HSP27和GRP78的組織分佈.結果 同基因植組,HSP27和GRP78在心髒組織內均勻分佈；異基因移植組,HSP27和GRP78在環血管週圍心肌組織中高錶達(2.24±0.36比1.04±0.10,P＜0.01；1.51±0.12比0.85±0.30,P＜0.05),而在增生的冠狀動脈組織內幾乎不錶達(2.24±0.36比0.25±0.08,P＜0.01；1.51±0.12比0.16±0.10,P＜0.01).結論 HSP27和GRP78在異基因移植後心髒組織中分佈不均,導緻冠狀動脈組織無力對抗免疫損傷,誘髮血管平滑肌細胞過度增殖可能是CAV髮生、髮展的重要原因.
목적 관찰이식후심장조직중열휴극단백27(HSP27)화당조절단백78(GRP78)적조직분포,탐토이식후심장혈관병변(CAV)적발병궤제.방법 건립대서동기인(n=8)화이기인(n=8)심장이식적Ono모형,채용면역조직화학법[련매균항생물소단백-과양화물매(SP)법]험증이식후심장조직중HSP27화GRP78적조직분포.결과 동기인식조,HSP27화GRP78재심장조직내균균분포；이기인이식조,HSP27화GRP78재배혈관주위심기조직중고표체(2.24±0.36비1.04±0.10,P＜0.01；1.51±0.12비0.85±0.30,P＜0.05),이재증생적관상동맥조직내궤호불표체(2.24±0.36비0.25±0.08,P＜0.01；1.51±0.12비0.16±0.10,P＜0.01).결론 HSP27화GRP78재이기인이식후심장조직중분포불균,도치관상동맥조직무력대항면역손상,유발혈관평활기세포과도증식가능시CAV발생、발전적중요원인.
Objective To observe the protein expression of heat shock protein 27 (HSP27) and glucose regulated protein 78 (GRP78) in rats transplanted hearts,and provide some clues to elucidate the pathogenesis of cardiac allograft vasculopathy (CAV).Methods The hearts were transplanted from Lewis to Sprague-Dawely rats as allograft (n =8) and from Lewis to Lewis rats as isograft (n =8) based on Ono' s model.The protein expression of HSP27 and GRP78 was detected by using immunohistochemistry [streptavid-inperoxidase (SP) method].Results The expression of HSP27 and GRP78 was stained uniformly in cardiac isografts,but that was anomalous in cardiac allografts,which showed higher expression on cardiac muscle (2.24 ± 0.36 vs.1.04 ± 0.10,P ＜ 0.01 ; 1.51 ± 0.12 vs.0.85 ± 0.30,P ＜ 0.05) and lower expression on blood vessels in biopsies with CAV (2.24 ±0.36 vs.0.25 ±0.08,P ＜0.01 ; 1.51 ±0.12 vs.0.16 ± 0.10,P ＜ 0.01).Conclusion The less expression of HSP27 and GRP78 on blood vessels decreased the defense to immunologic injury,which stimulated the over-proliferation of vascular smooth muscle cells to repair the vessels damage in heart allografts.Meanwhile,these protective reactions could incur the development of CAV in heart allografts.
