CAJ | 학술논문

目的研究术前输注高表达吲哚胺2,3-双加氧酶(IDO)的供鼠树突状细胞(DC)对同种小鼠心脏移植后排斥反应的抑制作用.方法通过携带IDO基因的腺病毒转染供鼠DC获得IDO+ DC,建立小鼠同种异位心脏移植模型,术前给予受鼠不同干预,设立对照组、DC组和IDO组,观察各组移植心存活时间,术后7d获取移植心组织行病理学检查,采用聚合酶链反应检测IDO mRNA的表达水平,采用蛋白质印迹法检测IDO蛋白的表达水平,应用流式细胞仪检测外周血T淋巴细胞的凋亡率.结果对照组、DC组和IDO组移植心的中位存活时间分别为7、7.5和17.5d,IDO组明显延长(P＜0.01).与对照组和DC组比较,IDO组移植心排斥反应病理分级明显降低(P＜0.01).IDO组受鼠外周血CD3+T淋巴细胞的凋亡率高达(46.50±5.02)％,较其他两组明显升高(P＜0.01),且移植心组织IDO表达水平亦明显增高(P＜0.01.结论术前向受鼠输注转染IDO基因的供鼠DC可以诱导受鼠外周血T淋巴细胞的凋亡,对小鼠心脏移植排斥反应具有明显的抑制作用.
목적 연구술전수주고표체신타알2,3-쌍가양매(IDO)적공서수돌상세포(DC)대동충소서심장이식후배척반응적억제작용.방법 통과휴대IDO기인적선병독전염공서DC획득IDO+ DC,건립소서동충이위심장이식모형,술전급여수서불동간예,설립대조조、DC조화IDO조,관찰각조이식심존활시간,술후7d획취이식심조직행병이학검사,채용취합매련반응검측IDO mRNA적표체수평,채용단백질인적법검측IDO단백적표체수평,응용류식세포의검측외주혈T림파세포적조망솔.결과 대조조、DC조화IDO조이식심적중위존활시간분별위7、7.5화17.5d,IDO조명현연장(P＜0.01).여대조조화DC조비교,IDO조이식심배척반응병리분급명현강저(P＜0.01).IDO조수서외주혈CD3+T림파세포적조망솔고체(46.50±5.02)％,교기타량조명현승고(P＜0.01),차이식심조직IDO표체수평역명현증고(P＜0.01.결론 술전향수서수주전염IDO기인적공서DC가이유도수서외주혈T림파세포적조망,대소서심장이식배척반응구유명현적억제작용.
Objective To investigate the effect of high expression of functional IDO trom donor DCs on transplantation rejection in mice heterotopic cardiac transplantation.Method Adenovirus vector containing IDO gene was used to infect donor (C57BL/6) DCs to obtain IDO+ DCs.Mouse heterotopic cardiac transplantation models were established (C57BL/6-BALB/c) and the following groups were set up:control group,DCs injection group and IDO+ DCs injection group.Survival time of the donor heart in all groups was observed.Meanwhile,donor hearts were harvested at 7 th day post-transplantation for different examinations,including pathological examination,mRNA expression of IDO by real-time PCR,and IDO protein expression by Western blotting.Peripheral blood of recipients was also harvested for T lymphocyte apoptosis rate examination by FACS.Result Cardiac allograft median survival time in control group,DCs injection group and IDO+ DCs injection group was 7,7.5,and 17.5 days respectively.IDO+ DCs treatment significantly prolonged the survival time of donor hearts (P＜0.01).Pathological grading was significantly decreased (P＜0.01).The CD3+ T lymphocyte apoptosis rate in peripheral blood of recipients in IDO+ DCs injection group was (46.50 + 5.02)％,significantly higher than the other two groups (P＜0.01).Both IDO mRNA and protein expression showed significant increase (P＜0.01).Conclusion The preoperative medication with IDO+ DCs injection to the recipients could induce T lymphocyte apoptosis and significantly suppress the rejection in mice heterotopic cardiac homotransplantation.