CAJ | 학술논문

目的探讨卵巢癌患者外周血Foxp3+调节性T细胞(Treg细胞)的变化.方法分别采用流式细胞术、实时定量反转录聚合酶链反应和酶联免疫吸附测定法检测46例卵巢癌患者(卵巢癌组)和46例健康体检者(对照组)外周血CD4+ Foxp3+Treg细胞百分率、Foxp3 mRNA表达水平以及血浆转化生长因子-β1(TGF-β1)水平,并进行比较.结果卵巢癌组CD4+ Foxp3+ Treg细胞百分率[(11.42±2.67)％]、Foxp3 mRNA表达水平(0.59±0.21)以及血浆TGF-β1水平[(35 580±7274)ng/L]与对照组[分别为(8.94±1.98)％、0.37±0.14、(28 610±5631)ng/L]相比显著增高,差异有统计学意义(P=0.0000).结论卵巢癌患者外周血CD4+ Foxp3+Treg细胞数量和/或功能异常,可能与卵巢癌的发生有关.
목적 탐토란소암환자외주혈Foxp3+조절성T세포(Treg세포)적변화.방법 분별채용류식세포술、실시정량반전록취합매련반응화매련면역흡부측정법검측46례란소암환자(란소암조)화46례건강체검자(대조조)외주혈CD4+ Foxp3+Treg세포백분솔、Foxp3 mRNA표체수평이급혈장전화생장인자-β1(TGF-β1)수평,병진행비교.결과 란소암조CD4+ Foxp3+ Treg세포백분솔[(11.42±2.67)％]、Foxp3 mRNA표체수평(0.59±0.21)이급혈장TGF-β1수평[(35 580±7274)ng/L]여대조조[분별위(8.94±1.98)％、0.37±0.14、(28 610±5631)ng/L]상비현저증고,차이유통계학의의(P=0.0000).결론 란소암환자외주혈CD4+ Foxp3+Treg세포수량화/혹공능이상,가능여란소암적발생유관.
Objective To investigate the changes of peripheral blood Foxp3+ regulatory T cell (Treg cell) in patients with ovarian cancer (OC).Methods In 46 patients with OC and 46 normal controls,the percentage of peripheral blood CD4+ Foxp3+ Treg cell was assessed by flow cytometry and Foxp3 mRNA level was detected by real-time quantitative reverse transcription polymerase chain reaction.The level of plasma.transforming growth factor β1 (TGF-ββ 1) was measured by enzyme linked immunosorbent assay.Results The percentages of CD4+ Foxp3+ Treg cell,Foxp3 mRNA and level of plasma TGF-β1 in patients with OC were statistically higher than those in normal controls [(11.42 ± 2.67)％ vs.(8.94 ± 1.98)％,0.59 ± 0.21 vs.0.37 ±0.14,(35 580 ±7274) ng/L vs.(28 610 ±5631) ng/L,P=0.0000].Conclusion The number and/or function of CD4+ Foxp3+ Treg cell in peripheral blood of patients with OC are abnormal,CD4+ Foxp3+Treg cell may participate in the occurrence of OC.