CAJ | 학술논문

目的评价伤害性刺激因素对丙泊酚麻醉时犬脊髓γ-氨基丁酸(GABA)分布的影响.方法 12～ 18月龄健康杂种犬16只,雌雄不拘,体重10～12 kg,采用随机数字表法,将其分为2组(n=8):伤害性刺激组(S组)和对照组(C组).静脉注射丙泊酚7 mg/kg进行麻醉诱导,插入气管导管,连接麻醉机行机械通气,右股动脉穿刺置管监测MAP和脉率,静脉输注丙泊酚70 mg·kg-1·h-1维持麻醉.S组于犬尾中部皮下注射5％福尔马林300μl,C组注射等容量生理盐水.于注射福尔马林(或生理盐水)前(T1)和注射福尔马林(或生理盐水)后(T2)记录MAP和脉率.持续静脉输注丙泊酚50 min时处死动物,取颈椎2,3节段脊髓组织,采用高效液相色谱法,分别检测脊髓不同区域(前角、背角、中间带、前索、后索、外侧索)GABA水平.结果与T2时相比,T2时S组MAP和脉率均升高(P＜0.05).C组脊髓不同区域(前角、背角、中间带、前索、后索、外侧索)GABA水平比较差异无统计学意义(P＞0.05).与C组相比,S组脊髓前角和背角GABA水平升高(P＜0.05),其它区域GABA水平差异无统计学意义(P＞0.05).结论伤害性刺激因素可引起丙泊酚麻醉时犬脊髓前角和背角GABA水平升高.
목적 평개상해성자격인소대병박분마취시견척수γ-안기정산(GABA)분포적영향.방법 12～ 18월령건강잡충견16지,자웅불구,체중10～12 kg,채용수궤수자표법,장기분위2조(n=8):상해성자격조(S조)화대조조(C조).정맥주사병박분7 mg/kg진행마취유도,삽입기관도관,련접마취궤행궤계통기,우고동맥천자치관감측MAP화맥솔,정맥수주병박분70 mg·kg-1·h-1유지마취.S조우견미중부피하주사5％복이마림300μl,C조주사등용량생리염수.우주사복이마림(혹생리염수)전(T1)화주사복이마림(혹생리염수)후(T2)기록MAP화맥솔.지속정맥수주병박분50 min시처사동물,취경추2,3절단척수조직,채용고효액상색보법,분별검측척수불동구역(전각、배각、중간대、전색、후색、외측색)GABA수평.결과 여T2시상비,T2시S조MAP화맥솔균승고(P＜0.05).C조척수불동구역(전각、배각、중간대、전색、후색、외측색)GABA수평비교차이무통계학의의(P＞0.05).여C조상비,S조척수전각화배각GABA수평승고(P＜0.05),기타구역GABA수평차이무통계학의의(P＞0.05).결론 상해성자격인소가인기병박분마취시견척수전각화배각GABA수평승고.
Objective To evaluate the effect of the noxious stimulation factor on γ-aminobutyric acid (GABA) distribution in dog spinal cord during propofol anesthesia.Methods Sixteen healthy mongrel dogs of both sexes,aged 12-18 months,weighing 10-12 kg,were randomly divided into 2 groups (n =8 each):noxious stimulation group (S group) and control group (C group).Anesthesia was induced with propofol 7 mg/kg.The animals were mechanically ventilated after tracheal intubation.Right femoral artery was cannulated for mean arterial pressure (MAP) and pulse rate monitoring.Anesthesia was maintained with propofol infusion at a constant rate of 70 mg· kg-1 · h-1.5 ％ formalin 300 μl was subcutaneously injected into the central region of tails in group S,while the equal volume of normal saline was injected instead of formalin in group C.MAP and pulse rate were recorded before injection of formalin or normal saline (T1) and after injection of formalin or normal saline (T2).The dogs were scarified by decapitation at 50 min of continuous propofol infusion and cervical 2-3 segments of the spinal cord were removed for determination of GABA level in different regions of the spinal cord (frontal horn,posterior horn,intermediate zone,frontal funiculus,posterior funiculus and lateral funiculus) by HPLC.Results MAP and pulse rate were significantly higher at T2 than at T1 in S group (P ＜ 0.05).There were no significant differences in GABA level among the different regions of the spinal cord in C group (P ＞ 0.05).Compared with C group,GABA level in the frontal horn and posterior horn was significantly increased (P ＜ 0.05),and no significant change was found in the other regions of the spinal cord in S group (P ＞ 0.05).Conclusion The noxious stimulation factor can induce an increase in GABA level in the frontal horn and posterior horn of dog spinal cord during propofol anesthesia.