CAJ | 학술논문

目的分析上海地区1366例浅部念珠菌病的致病菌种构成及分布情况.方法对1366例儿童与成人浅部念珠菌病的致病菌种进行菌种鉴定:采用科玛嘉平板、API20C AUX等鉴定手段常规鉴定;采用Pal平板、木糖同化试验、45℃生长试验鉴定都柏林念珠菌;采用分子鉴定方法鉴定近年发现的新种,包括Candida orthopsilosis、Candida metapsilosis、Candida fermentati、Candida nivariensis、Candida bracarensis.对致病菌种的构成及分布情况进行分析.结果 1366例浅部念珠菌病的主要致病菌为白念珠菌(占79.0％),排在2～4位的依次为近平滑念珠菌(9.5％)、热带念珠菌(2.9％)和季也蒙念珠菌(1.9％),致病株中包括念珠菌新种Candida orthopsilosis(2株)和Candida metapsilosis(4株);儿童患者与成人患者的致病菌种构成差异有统计学意义(x2=196.46,P＜ 0.01),其中非白念珠菌分离率分别为14.4％和45.8％.结论白念珠菌仍为浅部念珠菌病的主要致病菌;新种Candida orthopsilosis和Candida metapsilosis可引起浅部念珠菌病.与儿童病例相比,成人病例中非白念珠菌的分离比例明显上升.
목적 분석상해지구1366례천부념주균병적치병균충구성급분포정황.방법 대1366례인동여성인천부념주균병적치병균충진행균충감정:채용과마가평판、API20C AUX등감정수단상규감정;채용Pal평판、목당동화시험、45℃생장시험감정도백림념주균;채용분자감정방법감정근년발현적신충,포괄Candida orthopsilosis、Candida metapsilosis、Candida fermentati、Candida nivariensis、Candida bracarensis.대치병균충적구성급분포정황진행분석.결과 1366례천부념주균병적주요치병균위백념주균(점79.0％),배재2～4위적의차위근평활념주균(9.5％)、열대념주균(2.9％)화계야몽념주균(1.9％),치병주중포괄념주균신충Candida orthopsilosis(2주)화Candida metapsilosis(4주);인동환자여성인환자적치병균충구성차이유통계학의의(x2=196.46,P＜ 0.01),기중비백념주균분리솔분별위14.4％화45.8％.결론 백념주균잉위천부념주균병적주요치병균;신충Candida orthopsilosis화Candida metapsilosis가인기천부념주균병.여인동병례상비,성인병례중비백념주균적분리비례명현상승.
Objective To analyze the composition and distribution of pathogens from 1366 superficial candidiasis cases in Shanghai.Methods Candida species identification was carried out for 1366 adults or children with superficial candidiasis by using CHROMagar Candida plates,API20C AUX system,etc.Pal's agar,Xylose assimilation and the test for growth at 45 ℃ were utilized to differentiate Candida dubliniensis.Newly identified pathogenic Candida species including Candida orthopsilosis,Candida metapsilosis,Candida fermentati,Candida nivariensis and Candida bracarensis were differentiated by molecular biological methods.Finally,the composition and distribution of pathogens in superficial candidiasis cases were statistically analyzed.Results A total of 1366 Candida strains,included 2 Candida orthopsilosis strains and 4 Candida metapsilosis strains,were isolated from these cases.Among these isolates,Candida albicans predominated(79.0％),followed by Candida parapsilosis(9.5％),Candida tropicalis(2.9％)and Candida guilliermondii(1.9％).The composition of Candida species was significantly different between child and adult patients(x2 =196.46,P ＜ 0.01),with the isolation rate of non-albicans Candida species being 14.4％ and 45.8％ respectively in child and adult patients.Conclusions Candida albicans is still the dominant pathogen of superficial candidiasis.Candida orthopsilosis and Candida metapsilosis can cause superficial candidiasis.The isolation rote of non-albicans Candida species is higher in adult patients than in child patients.