中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
11期
825-827
,共3页
张丽%吴文娟%起珏%涂颖%顾华%何黎
張麗%吳文娟%起玨%塗穎%顧華%何黎
장려%오문연%기각%도영%고화%하려
目的 探讨95％长波紫外线(UVA)联合5％中波紫外线(UVB)照射对原代人表皮角质形成细胞(HEK)增殖的影响,为构建日光对人工皮肤光损伤模型提供实验依据.方法 用总剂量分别为0、2.5、5、10、20、30、40、60 J/cm2的紫外线(含95％UVA和5％UVB),分别照射体外培养的HEK,继续培养24 h后,用噻唑蓝(MTr)法检测细胞活力,用SPSS软件计算引起HEK半数损伤时的紫外线照射剂量.结果 8种剂量紫外线照射后,HEK相对抑制率分别为0、1.03％、6.60％、17.28％、31.28％、49.59％、59.67％、70.99％,当总照射剂量≥10 J/cm2时,HEK相对抑制率与对照组相比,差异有统计学意义(F=62.11,P＜0.05);HEK相对抑制率为50％时的紫外线照射总剂量为31.31 J/cm2.结论 随着紫外线照射剂量的加大,HEK增殖活力降低,31.31 J/cm2为HEK半数死亡的照射剂量.
目的 探討95％長波紫外線(UVA)聯閤5％中波紫外線(UVB)照射對原代人錶皮角質形成細胞(HEK)增殖的影響,為構建日光對人工皮膚光損傷模型提供實驗依據.方法 用總劑量分彆為0、2.5、5、10、20、30、40、60 J/cm2的紫外線(含95％UVA和5％UVB),分彆照射體外培養的HEK,繼續培養24 h後,用噻唑藍(MTr)法檢測細胞活力,用SPSS軟件計算引起HEK半數損傷時的紫外線照射劑量.結果 8種劑量紫外線照射後,HEK相對抑製率分彆為0、1.03％、6.60％、17.28％、31.28％、49.59％、59.67％、70.99％,噹總照射劑量≥10 J/cm2時,HEK相對抑製率與對照組相比,差異有統計學意義(F=62.11,P＜0.05);HEK相對抑製率為50％時的紫外線照射總劑量為31.31 J/cm2.結論 隨著紫外線照射劑量的加大,HEK增殖活力降低,31.31 J/cm2為HEK半數死亡的照射劑量.
목적 탐토95％장파자외선(UVA)연합5％중파자외선(UVB)조사대원대인표피각질형성세포(HEK)증식적영향,위구건일광대인공피부광손상모형제공실험의거.방법 용총제량분별위0、2.5、5、10、20、30、40、60 J/cm2적자외선(함95％UVA화5％UVB),분별조사체외배양적HEK,계속배양24 h후,용새서람(MTr)법검측세포활력,용SPSS연건계산인기HEK반수손상시적자외선조사제량.결과 8충제량자외선조사후,HEK상대억제솔분별위0、1.03％、6.60％、17.28％、31.28％、49.59％、59.67％、70.99％,당총조사제량≥10 J/cm2시,HEK상대억제솔여대조조상비,차이유통계학의의(F=62.11,P＜0.05);HEK상대억제솔위50％시적자외선조사총제량위31.31 J/cm2.결론 수착자외선조사제량적가대,HEK증식활력강저,31.31 J/cm2위HEK반수사망적조사제량.
Objective To observe the effect of ultraviolet irradiation comparising 95％ ultraviolet A (UVA)and 5％ ultraviolet B(UVB)on the proliferation of human epidermal keratinocytes(HEKs),in hope to offer a basis for the construction of a photodamaged skin model induced by sunlight.Methods HEKs were isolated from foreskin tissue and cultured in vitro.After several passages,the HEKs were irradiated with different doses(0,2.5,5,10,20,30,40,60 J/cm2)of UV comprising 95％ UVA and 5％ UVB.Methyl thiazolyl tetrazolium(MTT)assay was used to evaluate cell viability after 24 hours of additional culture.SPSS 17.0 software was used to calculate the median lethal dose(LD50)of ultraviolet radiation in HEKs.Results The proliferation of HEKs was inhibited by 0,1.03％,6.60％,17.28％,31.28％,49.59％,59.67％ and 70.99％ respectively after irradiation with UV of 0,2.5,5,10,20,30,40 and 60 J/cm2.A significant inhibition of cell proliferation was observed in HEKs irradiated with UV at a dose of no lower than 10 J/cm2 compared with unirradiated HEKs(F =62.11,P ＜ 0.05).The LD50 of UV in HEKs was 31.31 J/cm2.Conclusions Aas the dose of UV irradiation increases,the proliferative activity of HEKs decreases,with the LD50 of UV being 31.31 J/cm2.
