CAJ | 학술논문

目的探索人结肠癌细胞SW480转染4IgB7-H3后对糖基转移酶β3GnT8和细胞表面多聚乳糖胺糖链表达的影响。方法脂质体介导的重组表达载体pIRES2-EGFP/4IgB7-H3转染SW480细胞，筛选并获得稳定表达4IgB7-H3的亚克隆细胞株；RT-PCR、Western Blot检测β3GnT8的表达；流式细胞术检测多聚乳糖胺寡糖链的表达。结果成功获得稳定表达4IgB7-H3的亚克隆细胞株SW480/4IgB7-H3；转染4IgB7-H3的SW480细胞中β3GnT8和多聚乳糖胺糖链的表达均明显下调（P＜0.05）。结论人肠癌细胞中4IgB7-H3过表达能抑制β3GnT8的表达，进而抑制多聚乳糖胺糖链的表达。
목적탐색인결장암세포SW480전염4IgB7-H3후대당기전이매β3GnT8화세포표면다취유당알당련표체적영향。방법지질체개도적중조표체재체pIRES2-EGFP/4IgB7-H3전염SW480세포，사선병획득은정표체4IgB7-H3적아극륭세포주；RT-PCR、Western Blot검측β3GnT8적표체；류식세포술검측다취유당알과당련적표체。결과성공획득은정표체4IgB7-H3적아극륭세포주SW480/4IgB7-H3；전염4IgB7-H3적SW480세포중β3GnT8화다취유당알당련적표체균명현하조（P＜0.05）。결론인장암세포중4IgB7-H3과표체능억제β3GnT8적표체，진이억제다취유당알당련적표체。
Objective To explore the expressions of glycosyltransferaseβ3GnT8 and polylactosamine on cell surface in human colon cancer cell line SW480 transfected with 4IgB7-H3.Methods Liposome-mediated method transfected recombinant expression carrier pIRES2-EGFP/4IgB7-H3 into SW480 cells;RT-PCR,Western Blot detected the expression of 4IgB7-H3 andβ3GnT8 at the mRNA and protein levels;Flow cytometry detcted lactosamine oligosaccharide chain expression.Results 4IgB7-H3 recombinant expression vector was successfully constructed.Then stably transfected SW480 cell line was got.Both the expressions ofβ3GnT8 and poly lactose amine sugar chain decreased signiifcantly(P<0.05) after transfection.Conclusion 4IgB7-H3 overexpression in human colorectal cancer cells can inhibitβ3GnT8 production,and thereby inhibit poly galactosamine extension.