CAJ | 학술논문

藏羚羊是我国青藏高原的特有物种．为分析藏羚羊独特的遗传变异特征和核DNA遗传多样性，以5个微卫星标记对50只藏羚羊的等位基因多态性进行了研究．研究结果表明：5个微卫星标记共检测到92个等位基因，每个标记的平均等位基因数为18.4个（在16～23之间），平均有效等位基因数为11.1；5个微卫星标记的多态信息含量均在0.8573以上，为高度多态标记，其中L03标记的多态信息含量最高，达0.9372；各标记的观测杂合度在0.4898～0.9091之间，期望杂合度在0.8770～0.9504之间，平均期望杂合度为0.8990，属于高度杂合标记，遗传变异丰富．这些筛选出的多态性微卫星标记可应用于藏羚羊遗传多样性、遗传结构分析及遗传图谱的构建等工作．
장령양시아국청장고원적특유물충．위분석장령양독특적유전변이특정화핵DNA유전다양성，이5개미위성표기대50지장령양적등위기인다태성진행료연구．연구결과표명：5개미위성표기공검측도92개등위기인，매개표기적평균등위기인수위18.4개（재16～23지간），평균유효등위기인수위11.1；5개미위성표기적다태신식함량균재0.8573이상，위고도다태표기，기중L03표기적다태신식함량최고，체0.9372；각표기적관측잡합도재0.4898～0.9091지간，기망잡합도재0.8770～0.9504지간，평균기망잡합도위0.8990，속우고도잡합표기，유전변이봉부．저사사선출적다태성미위성표기가응용우장령양유전다양성、유전결구분석급유전도보적구건등공작．
Tibetan antelope(pantholops hodgsonii) ,also known as the chiru ,is endemic to the Qing-hai-Tibet Plateau. A total of 50 individual of Tibetan antelope were analyzed using five microsatellite markers. With an average of 18.4 alleles per locus(ranging from 16 to 23) ,92 alleles were identified at 5 microsatellite loci. The average effective number of allele was 11.1 ,and the polymorphism information content(PIC) of the 5 markers were more than 0.8573 ,which indicated a high polymorphism. With an val-ue of 0.9372 ,the highest PIC was observed at locus L03. The observed heterozygote (Ho) of the 5 mark-ers ranged from 0.4898 to 0.9091 ,and the expected heterozygote (He) varied from 0.8770 to 0.9504 (av-erage 0.8990). The results revealed a high level of genetic variation at 5 loci ,which suggested that those microsatellite markers could be applied in studies of genetic diversity ,genetic constructure and construc-tion of the genetic map in Tibetan antelope.