CAJ | 학술논문

为提高目前高致病性蓝耳病（HR—PRRS）灭活疫苗的免疫效果，将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍，再经灭活后用Sephamse 4 Fast Flow分子筛柱层析，收集完整的病毒粒子，加油佐剂制成纯化HP—PRRS灭活疫苗。纯化后疫苗的攻毒保护率达到415以上，血清抗体检测阳转率达86％以上，均高于同批病毒液制成的未纯化灭活疫苗，且差异显著。试验证明，HR—PRRS病毒液经浓缩纯化制成疫苗后，增强了免疫效果。
위제고목전고치병성람이병（HR—PRRS）멸활역묘적면역효과，장경Marc-145세포배양수획적HP-PRRS병독원액초려농축50배，재경멸활후용Sephamse 4 Fast Flow분자사주층석，수집완정적병독입자，가유좌제제성순화HP—PRRS멸활역묘。순화후역묘적공독보호솔체도415이상，혈청항체검측양전솔체86％이상，균고우동비병독액제성적미순화멸활역묘，차차이현저。시험증명，HR—PRRS병독액경농축순화제성역묘후，증강료면역효과。
To improve purification and protective potency of HP- PRRS inactivated vaccine, HP- PRRS vires multiplying and collected from Marc - 145 cells was concentrated 50 times and then inactivated. Complete virion is separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified in- activated vaccine. Protective potency of purified vaccine is above 4/5 and positive conversion rate of antibody is over 86 % that all higher than that of unpurified vaccine. The differences are significant. The experiment illustrated that vaccines through concentrating and purifying can enhanced immune efficacy and non- viral protein can be removed.