海南师范大学学报：自然科学版
海南師範大學學報：自然科學版
해남사범대학학보：자연과학판
Journal of Hainan Normal University:Natural Science
2012年
4期
421-424
,共4页
海南龙血树叶%总皂苷%纤维素酶%提取工艺%正交试验
海南龍血樹葉%總皂苷%纖維素酶%提取工藝%正交試驗
해남룡혈수협%총조감%섬유소매%제취공예%정교시험
The leaves of Dracaena cambodiana Pierre ex Gagn%Total saponin%cellulase%Extraction Technology%or-thogonal experiment
研究了酶解法提取海南龙血树叶总皂苷的工艺.采用酶解-乙醇浸提,以龙血树叶总皂苷的提取率作为评价指标,通过单因素试验和正交试验,确定提取海南龙血树叶总皂苷的最佳工艺.结果表明：最佳工艺条件是,酶质量浓度0.20 mg/mL,酶解温度50℃,pH=5.0,酶解时间为1.5 h,料液比1∶12.在此条件下,海南龙血树叶总皂苷提取率为4.13%.
研究瞭酶解法提取海南龍血樹葉總皂苷的工藝.採用酶解-乙醇浸提,以龍血樹葉總皂苷的提取率作為評價指標,通過單因素試驗和正交試驗,確定提取海南龍血樹葉總皂苷的最佳工藝.結果錶明：最佳工藝條件是,酶質量濃度0.20 mg/mL,酶解溫度50℃,pH=5.0,酶解時間為1.5 h,料液比1∶12.在此條件下,海南龍血樹葉總皂苷提取率為4.13%.
연구료매해법제취해남룡혈수협총조감적공예.채용매해-을순침제,이룡혈수협총조감적제취솔작위평개지표,통과단인소시험화정교시험,학정제취해남룡혈수협총조감적최가공예.결과표명：최가공예조건시,매질량농도0.20 mg/mL,매해온도50℃,pH=5.0,매해시간위1.5 h,료액비1∶12.재차조건하,해남룡혈수협총조감제취솔위4.13%.
The extraction technology for the total saponin from the leaves of Dracaena cambodiana Pierre ex C, agn by enzymatic hydrolysis was studied. By means of enzymatic hydrolysis and extraction by ethanol, the optimum extraction technology was determined by single factor and orthogonal experiment, with the use of the extraction rate of the total saponin of the leaves of Dracaena cambodiana Pierre ex Gagn as the assessment index. The results showed that: the optimum conditions is, mass concentration of cellulase was 0.20 mg/ml, temperature was 50 ℃, pH is 5.0, time is 1.5 hour and solid-liquid ratio is 1 : 12, the extraction efficiency is 4.13% in this condition.
