贵州林业科技
貴州林業科技
귀주임업과기
GUIZHOU FORESTRY SCIENCE AND TECHNOLOGY
2012年
4期
30-35
,共6页
刘明航%朱芳明%刘峥%张汉尧
劉明航%硃芳明%劉崢%張漢堯
류명항%주방명%류쟁%장한요
柳属%ITS序列%系统发育树%亲缘关系
柳屬%ITS序列%繫統髮育樹%親緣關繫
류속%ITS서렬%계통발육수%친연관계
Salicaceae%ITS sequences%Phylogenetic tree%Relationship
本文分析了垂柳、曲枝垂柳、旱柳和龙爪柳以及其他柳属植物属内种间系统发育关系。提取垂柳、曲枝垂柳和龙爪柳基因组DNA，对其内转录间隔区（ITS）序列进行PCR扩增和序列测定。采用BLAST方法将植物内转录间隔区（ITS）序列在GenBank中进行同源搜索，应用邻接距离距阵法，建立柳属共19种的ITS序列的系统发育树，并探讨了其亲缘关系。结果表明，垂柳、曲枝垂柳、旱柳和龙爪柳应属于同一种，其关系为种内变异水平，与经典分类方法中垂柳与旱柳为两个不同种存在分歧。
本文分析瞭垂柳、麯枝垂柳、旱柳和龍爪柳以及其他柳屬植物屬內種間繫統髮育關繫。提取垂柳、麯枝垂柳和龍爪柳基因組DNA，對其內轉錄間隔區（ITS）序列進行PCR擴增和序列測定。採用BLAST方法將植物內轉錄間隔區（ITS）序列在GenBank中進行同源搜索，應用鄰接距離距陣法，建立柳屬共19種的ITS序列的繫統髮育樹，併探討瞭其親緣關繫。結果錶明，垂柳、麯枝垂柳、旱柳和龍爪柳應屬于同一種，其關繫為種內變異水平，與經典分類方法中垂柳與旱柳為兩箇不同種存在分歧。
본문분석료수류、곡지수류、한류화룡조류이급기타류속식물속내충간계통발육관계。제취수류、곡지수류화룡조류기인조DNA，대기내전록간격구（ITS）서렬진행PCR확증화서렬측정。채용BLAST방법장식물내전록간격구（ITS）서렬재GenBank중진행동원수색，응용린접거리거진법，건립류속공19충적ITS서렬적계통발육수，병탐토료기친연관계。결과표명，수류、곡지수류、한류화룡조류응속우동일충，기관계위충내변이수평，여경전분류방법중수류여한류위량개불동충존재분기。
Phylogenetic relationships among species of Salix were revealed in this paper. Genomic DNA of Salix babylonica, Salix babylonica f. tortuosa and Sdix matsudana f. tortuosa were extracted, and their internal transcribed spacer region (ITS) sequences were amplified by PCR, and sequenced. The other ITS sequences of Salix species were acquired from the GenBank by the means of BLAST. Neighbor joining method was used to construct phylogenetic tree of those species, and genetic relationships among them were discussed. Sequences analysis indicated that Salix babyLonica, Salix babylonica f. tortuosa, Salix matsudana f. tortuosa and Salix matsudana actually belong to one species. The results are different with the classical classification that Salix babylonica and Salix matsudana are two species.
