CAJ | 학술논문

用不同有机酸-低温乙醇纯化腹水，确定纯化效果最好的有机酸为正己酸，纯化的IgM纯度可达95％，收率将近90％；用正己酸沉庭法配合低温乙醇沉淀法分别纯化不同抗原免疫的三种IgM型单抗，对多数IgM类抗体纯度可达到85％以上，IgM回收卒大于85％，表明这种纯化方法适用于多数IgM类单抗的纯化；用硫酸铵沉淀法及正己酸-低温乙醇沉淀法纯化相同的抗体，对活性都不影响，但正己酸-低温乙醇沉淀法纯化的抗体纯度远高于硫酸铵沉淀法、因此正己酸-低温乙醇沉淀法是一种简单、快速、温和的从小鼠腹水中纯化IgM方法，可满足一般实验的要求、
용불동유궤산-저온을순순화복수，학정순화효과최호적유궤산위정기산，순화적IgM순도가체95％，수솔장근90％；용정기산침정법배합저온을순침정법분별순화불동항원면역적삼충IgM형단항，대다수IgM류항체순도가체도85％이상，IgM회수졸대우85％，표명저충순화방법괄용우다수IgM류단항적순화；용류산안침정법급정기산-저온을순침정법순화상동적항체，대활성도불영향，단정기산-저온을순침정법순화적항체순도원고우류산안침정법、인차정기산-저온을순침정법시일충간단、쾌속、온화적종소서복수중순화IgM방법，가만족일반실험적요구、
It describes a separation of IgM from mouse ascites fluid by precipitation of caproic acid - cold ethanol precipitation method(CACE) and its effect was investigated. The results show that many other proteins are removed from serum by precipitati- on of caproic acid which is better then other. And a purity of 95% and yield almost to 90% is obtained followed by cold ethanol pre-cipitation method. Purification three IgM antibodies to different antigens with CACE the results show that the purity of the majority of IgM antibody can be up to above 85% and yield more than 80%, which means it is applicable to most of the mouse ascites fluid of IgM. Combination of the ammonium sulfate precipitation method (AS) and the CACE, the result of enzyme linked immunosorbent assay(EL1SA) indicates that any of the active of the IgM almost has no reduction compared with ascites. However, the purity of the IgM purified by CACE is much higher than the lgM purified by AS. This represents a simple, rapid and gentle approach to isolate lgM from mouse ascites fluid and can meet the general experimental requirements.