CAJ | 학술논문

　　目的探讨Fas通路的非凋亡信号在胃癌细胞株AGS中促进增殖转移的作用。方法流式细胞仪检测AGS细胞表面Fas及FasL表达情况；根据FasL不同作用浓度梯度，绘制细胞生长曲线；shRNA沉默AGS的Fas表达后，或NOK-1拮抗FasL后，利用transwell实验检测AGS的侵袭能力；免疫组化检测FasL表达与E-cadherin关系。结果低浓度的FasL（12.5 ng/ml）刺激对细胞增殖无明显抑制作用，反而能提高肿瘤细胞的侵袭能力。在沉默Fas受体或经NOK-1拮抗FasL后，该效应减弱。FasL和E-cadherin在胃癌组织中的表达呈负性相关。结论低剂量的FasL不能诱导AGS产生凋亡效应，反而通过Fas信号通路促进其侵袭转移能力。
　　목적탐토Fas통로적비조망신호재위암세포주AGS중촉진증식전이적작용。방법류식세포의검측AGS세포표면Fas급FasL표체정황；근거FasL불동작용농도제도，회제세포생장곡선；shRNA침묵AGS적Fas표체후，혹NOK-1길항FasL후，이용transwell실험검측AGS적침습능력；면역조화검측FasL표체여E-cadherin관계。결과저농도적FasL（12.5 ng/ml）자격대세포증식무명현억제작용，반이능제고종류세포적침습능력。재침묵Fas수체혹경NOK-1길항FasL후，해효응감약。FasL화E-cadherin재위암조직중적표체정부성상관。결론저제량적FasL불능유도AGS산생조망효응，반이통과Fas신호통로촉진기침습전이능력。
Objective To explore the non-apoptotic ability of Fas signaling pathway to promote the proliferation and the metastasis in gastric cancer cell line AGS. Methods Flow cytometry was used to detect AGS cell surface expression of Fas and FasL expression. According to the different role of FasL concentration gradient, we draw the cell growth curve. Transwell experiments were used to test the AGS invasion ability after silencing AGS Fas expression, or antagonsting the ability with NOK-1. Immunohistochemical assay was used to detcet FasL expression of E-cadherin. Results No significant inhibition and tumor cell invasion was found after low concentration of FasL (12.5 ng/ml) stimulation. In silenced Fas receptor or FasL antagonized by NOK-1 , the effect was weakend. The expression of FasL and E-cadherin in gastric cancer tissues showed a negative correlation. Conclusion Low doses of FasL failed to induce AGS produce apoptotic effects, but through Fas signaling pathway can promote its invasion and metastasis.