蚕桑通报
蠶桑通報
잠상통보
BULLETIN OF SERICULTURE
2012年
4期
19-22
,共4页
SSR分子标记%PCR%PAGE
SSR分子標記%PCR%PAGE
SSR분자표기%PCR%PAGE
SSR Molecular Marker%PCR%PAGE
利用公布的家蚕基因组序列，设计特定的家蚕微卫星引物，对8个不同品种家蚕的微卫星位点进行PCR扩增，所得PCR产物经变性与非变性聚丙烯凝胶（PAGE）分离，银染后发现在非变性PAGE上，条带较粗且模糊，非特异带较多，导致读带误差增大；而在变陛PAGE上，所得条带较细且比较清晰，非特异带明显减少，有利于降低统计误差。
利用公佈的傢蠶基因組序列，設計特定的傢蠶微衛星引物，對8箇不同品種傢蠶的微衛星位點進行PCR擴增，所得PCR產物經變性與非變性聚丙烯凝膠（PAGE）分離，銀染後髮現在非變性PAGE上，條帶較粗且模糊，非特異帶較多，導緻讀帶誤差增大；而在變陛PAGE上，所得條帶較細且比較清晰，非特異帶明顯減少，有利于降低統計誤差。
이용공포적가잠기인조서렬，설계특정적가잠미위성인물，대8개불동품충가잠적미위성위점진행PCR확증，소득PCR산물경변성여비변성취병희응효（PAGE）분리，은염후발현재비변성PAGE상，조대교조차모호，비특이대교다，도치독대오차증대；이재변폐PAGE상，소득조대교세차비교청석，비특이대명현감소，유리우강저통계오차。
Silkworm SSR primers were designed based on the silkworm genome sequence. SSR products were amplified from 8 silkworm varieties, and detected by denatured and non-denatured gels respectively. In non-denatured gel, there were many nonspecific bands and shadow bands, leading to misread of amplication. In denatured gel, nonspecific and shadow bands were greatly lessened while clear purposed bands were showed, in favor of decreasing the statistical error.
