CAJ | 학술논문

目的：观察血余炭纳米纤维膜外用对皮肤的刺激性和过敏性反应，为评价其安全性提供参考。方法：采用家兔皮肤刺激实验，分完整皮肤组和损伤皮肤组，分别在给药1d、连续给药7d后，观察皮肤有无红斑、水肿等刺激反应；采用豚鼠皮肤过敏实验，每周贴敷血余炭纳米纤维膜致敏1次，连续3周，于首次致敏后的第28天皮肤再次给药进行攻击，观察皮肤有无红斑、水肿等过敏反应。结果：血余炭纳米纤维膜对家兔的完整皮肤和破损皮肤，经单次给药或多次给药24 h后去除敷料,分别于1、24、48、72 h观察，均未见红斑及水肿，刺激反应平均分值均<0.5，无刺激性；豚鼠皮肤过敏实验中，未出现皮肤明显红斑、水肿等皮肤过敏反应及其他全身性过敏反应。结论：血余炭纳米纤维膜对家兔完整皮肤和损伤皮肤不会引起刺激性反应，对豚鼠皮肤无过敏性反应，具有良好的皮肤用药安全性。
목적：관찰혈여탄납미섬유막외용대피부적자격성화과민성반응，위평개기안전성제공삼고。방법：채용가토피부자격실험，분완정피부조화손상피부조，분별재급약1d、련속급약7d후，관찰피부유무홍반、수종등자격반응；채용돈서피부과민실험，매주첩부혈여탄납미섬유막치민1차，련속3주，우수차치민후적제28천피부재차급약진행공격，관찰피부유무홍반、수종등과민반응。결과：혈여탄납미섬유막대가토적완정피부화파손피부，경단차급약혹다차급약24 h후거제부료,분별우1、24、48、72 h관찰，균미견홍반급수종，자격반응평균분치균<0.5，무자격성；돈서피부과민실험중，미출현피부명현홍반、수종등피부과민반응급기타전신성과민반응。결론：혈여탄납미섬유막대가토완정피부화손상피부불회인기자격성반응，대돈서피부무과민성반응，구유량호적피부용약안전성。
Objective:To evaluate the safety of the nano-fibers tunic carbonized hair loaded drug (NTCH) by skin irritation and anaphylaxis experiment. Methods: Skin irritation test was carried out on the integral skin and the damaged skin of rabbits, which was observed at 1 day and 7 days with the NTCH. Skin allergy test was carried out on the slain of the guinea pigs with NTCH once a week for 3 consecutive weeks, and the animals were stimulated at the 28th day. Then irritation and allergic reactions of the animals were investigated. Results:There was no erythema and edema at 1 h, 24 h, 48 h and 72 h after single dose or multiple doses of NTCH. The average reaction values of the NTCH on integral and damaged skin were lower than 0.5, and there was no irritation of NTCH on integral and damaged skin of rabbits. There was no allergic reactions such as erythema and edema or systemic allergic reactions in guinea pigs skin test. Conclusion:The NTCH is a safe external drug without irritation on integral and damaged skin of rabbit and anaphylaxis effect on the skin of guinea pig.