CAJ | 학술논문

目的：测定试验用抗菌药物与氟康唑联合的动态抗真菌作用，并评价试验用抗菌药物对氟康唑透过生物膜的影响。方法：采用活菌计数法描绘联合用药对白色念珠菌的动态杀菌曲线，以“三明治”透膜实验法评价试验用抗菌药物对氟康唑透过生物膜的影响。结果：试验抗菌药物对氟康唑有不同程度的增效作用，其中米诺环素和利福平对氟康唑透过耐药白色念珠菌生物膜的作用及其对氟康唑动态抗真菌的增效作用最强，氧氟沙星、阿奇霉素等次之。结论：试验用抗菌药物能促进氟康唑透过白色念珠菌生物膜，增强氟康唑的动态抗真菌作用。
목적：측정시험용항균약물여불강서연합적동태항진균작용，병평개시험용항균약물대불강서투과생물막적영향。방법：채용활균계수법묘회연합용약대백색념주균적동태살균곡선，이“삼명치”투막실험법평개시험용항균약물대불강서투과생물막적영향。결과：시험항균약물대불강서유불동정도적증효작용，기중미낙배소화리복평대불강서투과내약백색념주균생물막적작용급기대불강서동태항진균적증효작용최강，양불사성、아기매소등차지。결론：시험용항균약물능촉진불강서투과백색념주균생물막，증강불강서적동태항진균작용。
Objective:To determine the combined dynamic antifungal effects of fluconazole and the tested antibacterial agents and evaluate the impact of the tested antibacterial agents on fluconazole penetration through biofilm. Methods:The dynamic killing curve was obtained based on the combined drugs against Candida albicans using viable bacterial counting method. The biofilm penetration test was evaluated by “sandwich” experimental method. Results:Synergistic effects on fluconazole were showed by using the tested antibacterial agents. The penetrating ability of fluconazole through biofilm by the drug resistant Candida albicans varied by different tested antibacterial drugs ,of which minocycline and rifampicin shown strongest impact on it but also on the synergistic effects of dynamic antifungal action. Levofloxacin ,azithromycin and others had a lower impact. Conclusion:The tested antibacterial agents can promote the fluconazole penetration of Candida albican ’ s biofilm and increase fluconazole’s dynamic antifungal effect.