吉首大学学报:自然科学版
吉首大學學報:自然科學版
길수대학학보:자연과학판
Journal of Jishou University(Natural Science Edition)
2012年
5期
78-81
,共4页
红檵木%抑菌活性%最小抑菌生药浓度(MIC)
紅檵木%抑菌活性%最小抑菌生藥濃度(MIC)
홍계목%억균활성%최소억균생약농도(MIC)
L. chinense Var. rubrum%antibaterial action%minimal inhibition concentration of crude drug (MIC)
用蒸馏水和20%乙醇经微波辅助处理提取红檵木幼叶成分,对该水和醇水的提取物进行浓缩或萃取精制,采用牛津杯法测定水和醇水提取物样液对3种细菌的抑制效果,并用平板稀释法测定其最小抑菌浓度.幼叶水提取液对3种细菌都有明显的抑制作用,抑菌圈较大,对大肠杆菌、金黄色葡萄球菌和痢疾杆菌的最小抑菌生药质量浓度分别为0.014,0.010,0.010g/mL!幼叶的醇提取液对大肠杆菌的抑制效果不明显,对金黄色葡萄球菌和痢疾杆菌有较强抑制作用,其最小抑菌生药质量浓度分别为0.011,0.014g/mL.
用蒸餾水和20%乙醇經微波輔助處理提取紅檵木幼葉成分,對該水和醇水的提取物進行濃縮或萃取精製,採用牛津杯法測定水和醇水提取物樣液對3種細菌的抑製效果,併用平闆稀釋法測定其最小抑菌濃度.幼葉水提取液對3種細菌都有明顯的抑製作用,抑菌圈較大,對大腸桿菌、金黃色葡萄毬菌和痢疾桿菌的最小抑菌生藥質量濃度分彆為0.014,0.010,0.010g/mL!幼葉的醇提取液對大腸桿菌的抑製效果不明顯,對金黃色葡萄毬菌和痢疾桿菌有較彊抑製作用,其最小抑菌生藥質量濃度分彆為0.011,0.014g/mL.
용증류수화20%을순경미파보조처리제취홍계목유협성분,대해수화순수적제취물진행농축혹췌취정제,채용우진배법측정수화순수제취물양액대3충세균적억제효과,병용평판희석법측정기최소억균농도.유협수제취액대3충세균도유명현적억제작용,억균권교대,대대장간균、금황색포도구균화이질간균적최소억균생약질량농도분별위0.014,0.010,0.010g/mL!유협적순제취액대대장간균적억제효과불명현,대금황색포도구균화이질간균유교강억제작용,기최소억균생약질량농도분별위0.011,0.014g/mL.
The pigment in the browse leaves was extracted from L. Chinese Var. Rubrum leaf with Microwave treatment and distilled water or 20% EtOH. The antibacterial activities of the extracts on the three kinds bacteria were determined by Oxford Cup method. If the extracts can abstrain the bacteria obviously,the MIC of crude drug were determined by microdilution technique. The results show that the extracts from browse leaves by distilled water under microwave treatment have obviously antimicrobial activity on three kinds bacteria,the MIC of crude drug of the extracts from browse leaves on E. coli,shigella dysenteroae,staphylococlus aureus are 0. 014 g/mL,0. 010 g/mL,and 0. 010 g/mL. The extracts from browse leaves by 20 % EtOH were obviously active against staphylococlus aureus and shigella dysenteroae but can not abstrain the E. coli.