CAJ | 학술논문

以5个甘蓝品种为试材，从基因型以及培养基中的有机成分、蔗糖浓度和激素浓度4个方面，对利用生物技术创造甘蓝育种新材料的方法进行了研究。结果表明：不同品种之间的遗传差异影响了参试甘蓝花药胚状体的诱导频率；培养基中的有机成分、蔗糖浓度和激素浓度均对胚状体诱导频率有较大影响。并建立了1套通过甘蓝花粉培养诱导产生单倍体植株的技术：取未授粉甘蓝花药和花粉，接种在蔗糖浓度为10％、附加NAA0．2mg／L＋6-BA2mg／L的MS培养基中，诱导胚状体产生及萌发；将胚状体接种在附加NAA0．2mg／L的MS培养基中进行生根诱导，30d后生根率达89％，且根数较多，平均根长1．0～2．0cm，芽苗生长健壮。利用该技术获得单倍体再生植株所需要的时间一般为80d左右。
이5개감람품충위시재，종기인형이급배양기중적유궤성분、자당농도화격소농도4개방면，대이용생물기술창조감람육충신재료적방법진행료연구。결과표명：불동품충지간적유전차이영향료삼시감람화약배상체적유도빈솔；배양기중적유궤성분、자당농도화격소농도균대배상체유도빈솔유교대영향。병건립료1투통과감람화분배양유도산생단배체식주적기술：취미수분감람화약화화분，접충재자당농도위10％、부가NAA0．2mg／L＋6-BA2mg／L적MS배양기중，유도배상체산생급맹발；장배상체접충재부가NAA0．2mg／L적MS배양기중진행생근유도，30d후생근솔체89％，차근수교다，평균근장1．0～2．0cm，아묘생장건장。이용해기술획득단배체재생식주소수요적시간일반위80d좌우。
Using 5 cabbage varieties as materials, the method of creation of cabbage breeding new materials by biological technology was studied from four aspects of genotype and organic component, sucrose concentration and hormone concentration in the culture medium. The results showed that induction frequency of anther embryoids of tested cabbage was affected by genetic differences of different varieties. Organic component, sucrose concentration and hormone concentration in the culture medium had great influences on embryoid induction frequency. A set of technology of induced haploid plants by cabbage pollen culture was established, the unpollinated anthers and pollen of cabbage were nurtured in MS medium with 10% of sucrose concentration and adding NAA 0.2 mg/L + 6-BA 2 mg/L to induce embryoids generation and germination, then the embryoids were inoculated on the MS medium with NAA 0. 2 mg/L for 30 days to root induction, and the rate of rooting reached to 89% , the number of roots was large, the average root length was 1.0 - 2.0 cm, bud seedling growed healthy. The time required to obtain haploid regenerated plants was generally about 80 days by the technology.