CAJ | 학술논문

在改进的CTAB提取法基础上，结合总RNA的LiCl沉淀法，摸索出了一套适合苹果组织总RNA提取和纯化的方法。该方法得到的苹果总RNA完整性好，纯度和得率高，且成本低，容易操作。解决了RNA易降解以及木本植物组织中多酚和多糖含量高导致的在提取过程中RNA被氧化和共沉淀从而导致RNA产率低的问题。
재개진적CTAB제취법기출상，결합총RNA적LiCl침정법，모색출료일투괄합평과조직총RNA제취화순화적방법。해방법득도적평과총RNA완정성호，순도화득솔고，차성본저，용역조작。해결료RNA역강해이급목본식물조직중다분화다당함량고도치적재제취과정중RNA피양화화공침정종이도치RNA산솔저적문제。
Based on the improvement of CTAB extraction method, and combining the LiCL precipitation methed of total RNA, a set of method suitable for apple tatol RNA extraction and purification was built, which was simple and low-cost, and the total RNA received with high-quality and high-quantity. The problems of RNA degradation and low yield of RNA attributed to oxidation by polyphenol and coprecipitation with polysaccharides were solved.