CAJ | 학술논문

目的：优选滁菊总黄酮提取纯化工艺条件。方法：以滁菊总黄酮含量为考察指标，采用正交试验设计法优选滁菊总黄酮提取的工艺条件；比较D-101型大孔树脂优选滁菊总黄酮纯化的工艺条件。结果：最佳提取工艺条件为70％乙醇，料液比l：20，提取时间为1．5h，提取温度为60℃；最佳纯化工艺条件为上样液浓度29．75mg／mL，70％乙醇为洗脱剂，流速1BV／h，洗脱剂用量120mL。结论：本方法可以作为滁菊总黄酮提取和纯化的工艺条件。
목적：우선저국총황동제취순화공예조건。방법：이저국총황동함량위고찰지표，채용정교시험설계법우선저국총황동제취적공예조건；비교D-101형대공수지우선저국총황동순화적공예조건。결과：최가제취공예조건위70％을순，료액비l：20，제취시간위1．5h，제취온도위60℃；최가순화공예조건위상양액농도29．75mg／mL，70％을순위세탈제，류속1BV／h，세탈제용량120mL。결론：본방법가이작위저국총황동제취화순화적공예조건。
Objective:To optimize the extraction and purification technology of total flavonoids in Chu chrysanthe- mum. Methods: Single factor and orthogonal experiment design were used to optimize the extraction technology of total flavonoids in Chu chrysanthemum ; D - 101 resin was used to optimize the purification technology of total fla- vonoids in Chu chrysanthemum. Results :The optimum extraction conditions are 70% alocohol, 1:20 dosage liq- uor ratio ,1.5h extraction time,60℃ extraction temperature; The optimum purification condition are 29.75mg/ mL sample concentration, 70% eluting reagent concentration, 1BV/h flow rate, 120mL eluting reagent dosage. Conclusion:The condition can be used as extraction and purification technology of total flavonoids in Chu chrys- anthemum.