园艺与种苗
園藝與種苗
완예여충묘
Rain Fed Crops
2012年
12期
36-39
,共4页
二乔杜鹃%生根培养%正交试验
二喬杜鵑%生根培養%正交試驗
이교두견%생근배양%정교시험
Rhododendron spp. cv. Erqiaodujuan%Rooting culture%Orthogonal experiments
[目的】对二乔杜鹃（Rhododendron spp.ev.Erqiaodujuan）组培苗生根培养进行优化分析。【方法】以二乔杜鹃组培苗为材料，通过正交试验法研究基本培养基、NAA、IBA和蔗糖对二乔杜鹃组培苗生根培养的影响。【结果】二乔杜鹃生根培养的优化培养基为1／4WPM＋NAA1．5mg／1．＋IBA0．5mg／L＋蔗糖10g／L＋琼脂6g／L，生根率可达89．50％；移栽后试管苗正常生长。【结论】该研究可为二乔杜鹃的快繁提供科学依据，并为其他名贵杜鹃和濒危杜鹃等的繁育、保护和利用提供技术参考。
[目的】對二喬杜鵑（Rhododendron spp.ev.Erqiaodujuan）組培苗生根培養進行優化分析。【方法】以二喬杜鵑組培苗為材料，通過正交試驗法研究基本培養基、NAA、IBA和蔗糖對二喬杜鵑組培苗生根培養的影響。【結果】二喬杜鵑生根培養的優化培養基為1／4WPM＋NAA1．5mg／1．＋IBA0．5mg／L＋蔗糖10g／L＋瓊脂6g／L，生根率可達89．50％；移栽後試管苗正常生長。【結論】該研究可為二喬杜鵑的快繁提供科學依據，併為其他名貴杜鵑和瀕危杜鵑等的繁育、保護和利用提供技術參攷。
[목적】대이교두견（Rhododendron spp.ev.Erqiaodujuan）조배묘생근배양진행우화분석。【방법】이이교두견조배묘위재료，통과정교시험법연구기본배양기、NAA、IBA화자당대이교두견조배묘생근배양적영향。【결과】이교두견생근배양적우화배양기위1／4WPM＋NAA1．5mg／1．＋IBA0．5mg／L＋자당10g／L＋경지6g／L，생근솔가체89．50％；이재후시관묘정상생장。【결론】해연구가위이교두견적쾌번제공과학의거，병위기타명귀두견화빈위두견등적번육、보호화이용제공기술삼고。
[Objective] The aim was to optimize rooting conditions of Rhododendron spp.ev.Erqiaodujuan.[Method] Using the tube plantlets of Rhododendron spp.ev.Erqiaodujuan as the experimental materials, the effects of the basal media, concentrations of NAA, IBA anti sucrose of rooting conditions were analyzed by orthogonal experiments. [Resultl The results showed that the best rooting medium was 1/4 WPM + 1.5 mg/L NAA + 0.5 mg/L IBA + 10 g/L sucrose + 6 g/L agar and the rooting rate was as high as 89.50%. After transplanl, the plantlets grew normally. [Conclusion]The study provided a scientific basis for tire micropropagation of Rhododendron spp.ev.Erqiaodujuan, and also provided a lechnical reference for propagation, protection and utilization in other valuable or endangered Rhododendron species.
