CAJ | 학술논문

对云南7个优质主产烟区收集的450份烤后烟样，运用4％NaCl培养基选择分离法，分离出苏云金芽孢杆菌475株。其中对含毒蛋白基因cryⅠ及cryⅤ的18个Bt菌株用2龄烟草甲虫进行生物毒力测定，试验9d后，9个Bt菌株生物毒力测定校正死亡率均超过80％；试验12d后．5个Bt菌株生物毒力测定校正死亡率均超过95％。同时对分离出的Bt菌进行PCR毒素蛋白基因鉴定，舍cryⅠ毒素蛋白基因的Bt菌有7株，出菌率为1．47％；含cryⅤ毒素蛋白基因的Bt菌11株，出菌率为2．32％；没有含cryⅢ毒素蛋白基因的Bt菌。因此，为进一步用苏云金芽孢杆菌防治烟叶仓贮害虫提供了科学依据。
대운남7개우질주산연구수집적450빈고후연양，운용4％NaCl배양기선택분리법，분리출소운금아포간균475주。기중대함독단백기인cryⅠ급cryⅤ적18개Bt균주용2령연초갑충진행생물독력측정，시험9d후，9개Bt균주생물독력측정교정사망솔균초과80％；시험12d후．5개Bt균주생물독력측정교정사망솔균초과95％。동시대분리출적Bt균진행PCR독소단백기인감정，사cryⅠ독소단백기인적Bt균유7주，출균솔위1．47％；함cryⅤ독소단백기인적Bt균11주，출균솔위2．32％；몰유함cryⅢ독소단백기인적Bt균。인차，위진일보용소운금아포간균방치연협창저해충제공료과학의거。
475 Bacillus thuringiensis strains were isolated from 450 tobacco sample collected from 7 main tobacco-produced area in Yunnan by the method of 4% NaCI cutural medium and identification of them were done by means of PCR toxin gene.The results indicated that 7 Bt strains which plating efficiency was 1.47% contained cry I toxin gene, 11 Bt strains contained cry V gene which plating efficiency was 2.32% ,there was no Bt isolates containing cry Ⅱ gene.The bioassay toxicity of 18 Bt isolates containing cry Ⅰ and cry Ⅴ gene was determinated to Lasioderma serricorne (F.) second instar, the results showed that there existed all of 9 Bt with over 80% corrected mortality 9 days after inoculation and 12 days after that 5 isolates with over 95% corrected mortality.From these the conclusion can be drawn that Bt is potential by used to control pest on stored tobacco leaf.