药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2014年
2期
115-117
,共3页
复方桔梗止咳片%高效液相色谱法%甘草苷%甘草酸%含量测定
複方桔梗止咳片%高效液相色譜法%甘草苷%甘草痠%含量測定
복방길경지해편%고효액상색보법%감초감%감초산%함량측정
Compound radix platycodi cough tablets%Licorice glycosides%Glycyrrhizic acid%Content de-termination%Assay
目的：建立同时测定复方桔梗止咳片中甘草苷和甘草酸含量的HPLC检测方法。方法：采用Agilent C18（4.6 mm×150 mm，5μm）色谱柱；以乙腈-0.05%磷酸为流动相进行梯度洗脱；流速1.0 mL·min-1；检测波长237 nm；进样量：10μL；柱温30℃。结果：甘草苷、甘草酸分别在10.65～42.60 mg·L-1、97.30～389.30 mg·L-1范围内呈良好的线性关系，r分别为1.0000、1.0000；平均回收率（n=6）分别为98.80%（RSD=0.73%）、99.84%（RSD=0.10%）。测得6批复方桔梗止咳片，每片分别含甘草苷135.28μg、123.16μg、113.21μg、136.08μg、160.84μg、156.32μg，甘草酸441.36μg、405.86μg、364.69μg、446.93μg、518.22μg、509.65μg。结论：该方法简便，重复性好，可用于同时测定复方桔梗止咳片中甘草苷和甘草酸的含量。
目的：建立同時測定複方桔梗止咳片中甘草苷和甘草痠含量的HPLC檢測方法。方法：採用Agilent C18（4.6 mm×150 mm，5μm）色譜柱；以乙腈-0.05%燐痠為流動相進行梯度洗脫；流速1.0 mL·min-1；檢測波長237 nm；進樣量：10μL；柱溫30℃。結果：甘草苷、甘草痠分彆在10.65～42.60 mg·L-1、97.30～389.30 mg·L-1範圍內呈良好的線性關繫，r分彆為1.0000、1.0000；平均迴收率（n=6）分彆為98.80%（RSD=0.73%）、99.84%（RSD=0.10%）。測得6批複方桔梗止咳片，每片分彆含甘草苷135.28μg、123.16μg、113.21μg、136.08μg、160.84μg、156.32μg，甘草痠441.36μg、405.86μg、364.69μg、446.93μg、518.22μg、509.65μg。結論：該方法簡便，重複性好，可用于同時測定複方桔梗止咳片中甘草苷和甘草痠的含量。
목적：건립동시측정복방길경지해편중감초감화감초산함량적HPLC검측방법。방법：채용Agilent C18（4.6 mm×150 mm，5μm）색보주；이을정-0.05%린산위류동상진행제도세탈；류속1.0 mL·min-1；검측파장237 nm；진양량：10μL；주온30℃。결과：감초감、감초산분별재10.65～42.60 mg·L-1、97.30～389.30 mg·L-1범위내정량호적선성관계，r분별위1.0000、1.0000；평균회수솔（n=6）분별위98.80%（RSD=0.73%）、99.84%（RSD=0.10%）。측득6비복방길경지해편，매편분별함감초감135.28μg、123.16μg、113.21μg、136.08μg、160.84μg、156.32μg，감초산441.36μg、405.86μg、364.69μg、446.93μg、518.22μg、509.65μg。결론：해방법간편，중복성호，가용우동시측정복방길경지해편중감초감화감초산적함량。
Objective: To establish a quantitative method for the determination of licorice glycosides and glycyrrhizic acid in the compound radix platycodi cough tablets by high-performance liquid chromatography (HPLC) coupled with ultraviolet detection. Methods: An Agilent C18 column (4.6 mm ×150 mm, 5μm) was adopted. The mobile phase consisted of a mixture of acetonitrile - 0.05% phosphoric acid in gradient elution at a flow rate of 1.0 mL·min-1, the detection wavelength was set at 237 nm; The sampling volume was 10μL; The column temperature was 30℃. Results: The calibration curve showed a good linearity in the range of 10.65~42.60 mg·L-1 (r=1.0000) for licorice glycosides and 97.30~389.30 mg·L-1 (r=1.0000) for glycyrrhizic acid, respectively. The average recoveries (n=6) of licorice glycosides and glycyrrhizic acid were 98.80% (RSD=0.73%) and 99.84% (RSD=0.10%), respectively. Six batches of compound radix platycodi cough tablets were measured and the content per pill was respectively as follows: liquorice glycosides, 135.28μg, 123.16μg, 113.21μg, 136.08μg, 160.84μg and 156.32μg; glycyrrhizic acid, 441.36μg, 405.86μg, 364.69μg, 446.93μg, 518.22μg and 509.65μg. Conclusions: The developed method is simple and reproducible, which can be used for the quantitative determination of licorice glycosides and glycyrrhizic acid in the compound radix platycodi cough tablets.
