CAJ | 학술논문

运用基因组步移技术分离获得了CaKR1基因上游-1594 bp的启动子序列，命名为CaKR1p，发现其中含有SA、ABA、低温等信号应答原件以及其它诸如W盒等应答逆境胁迫的调控原件。进一步构建CaKR1p与GUS报告基因融合的植物表达载体，获得了烟草转基因植株及其相应的T1代株系，利用T1代转基因株系分析了CaKR1p在几种外源激素处理下的GUS基因的表达，结果表明外源激素SA、JA和ABA的诱导处理均可激活该启动子下游报告基因GUS的表达，说明CaKR1的表达和作用受到SA、ABA以及JA等信号通路调节。
운용기인조보이기술분리획득료CaKR1기인상유-1594 bp적계동자서렬，명명위CaKR1p，발현기중함유SA、ABA、저온등신호응답원건이급기타제여W합등응답역경협박적조공원건。진일보구건CaKR1p여GUS보고기인융합적식물표체재체，획득료연초전기인식주급기상응적T1대주계，이용T1대전기인주계분석료CaKR1p재궤충외원격소처리하적GUS기인적표체，결과표명외원격소SA、JA화ABA적유도처리균가격활해계동자하유보고기인GUS적표체，설명CaKR1적표체화작용수도SA、ABA이급JA등신호통로조절。
In the present study , the -1594 bp upstream promoter sequence of gene CaKR1 was isolated by using genome walk-ing technology and named CaKR1p, and cis-elements such as SARE, ABRE, LTRE were found in this promoter.The promoter was fused to GUS reporter gene to generate plant expression vector .This expression vector was transformed into tobacco plants by u-sing Agrobacterium-mediated method , and the transgenic tobacco plants were acquired and confirmed .With T1 transgenic tobacco lines, we analyzed the expression of CaKR1p against the treatments of several exogenous hormones , and found that the expression of GUS reporter gene was induced significantly by exogenously -applied ABA, JA and SA, suggesting that the expression and function of CaKR1 are regulated by the signal paths of SA , ABA, JA and so on.