CAJ | 학술논문

目的：观察 miR -374过表达对人乳腺癌 MDA - MB -231细胞 docetaxel（多西他赛）耐药性的影响。方法：qPCR 检测 miR -374过表达细胞 MDA - MB -231- miR -374和对照细胞 MDA - MB -231- Cherry 中miR -374的相对含量；采用不同浓度的多西他赛处理细胞72小时后，MTT 检测细胞的活性。在终浓度为3.2 nmol/ L 的多西他赛处理下，用平板克隆实验检测 MDA - MB -231- Cherry 和 MDA - MB -231- miR -374细胞的增殖能力。流式细胞术检测细胞周期和凋亡。结果：与 MDA - MB -231- Cherry 细胞相比，miR -374过表达细胞 MDA - MB -231- miR -374中 miR -374的相对表达量明显增高；MDA - MB -231- miR -374细胞的耐药性和克隆形成能力明显增强（P ﹤0.05）。细胞凋亡率明显降低，G0/ G1期细胞减少，S 期细胞增加，G2/M 期细胞增加。结论：miR -374过表达可明显增强乳腺癌细胞株 MDA - MB -231对多西他赛的耐药性。
목적：관찰 miR -374과표체대인유선암 MDA - MB -231세포 docetaxel（다서타새）내약성적영향。방법：qPCR 검측 miR -374과표체세포 MDA - MB -231- miR -374화대조세포 MDA - MB -231- Cherry 중miR -374적상대함량；채용불동농도적다서타새처리세포72소시후，MTT 검측세포적활성。재종농도위3.2 nmol/ L 적다서타새처리하，용평판극륭실험검측 MDA - MB -231- Cherry 화 MDA - MB -231- miR -374세포적증식능력。류식세포술검측세포주기화조망。결과：여 MDA - MB -231- Cherry 세포상비，miR -374과표체세포 MDA - MB -231- miR -374중 miR -374적상대표체량명현증고；MDA - MB -231- miR -374세포적내약성화극륭형성능력명현증강（P ﹤0.05）。세포조망솔명현강저，G0/ G1기세포감소，S 기세포증가，G2/M 기세포증가。결론：miR -374과표체가명현증강유선암세포주 MDA - MB -231대다서타새적내약성。
Objective:To investigate the effects of miR - 374 overexPression on docetaxel drug resistance of breast cancer cell line MDA - MB - 231. Methods:The relative exPressions of miR - 374 in MDA - MB - 231 - Cherry and MDA - MB - 231 - miR - 374 cell lines were identified by qPCR. The cells were Planted into 96 - well Plates and treated with different concentration of docetaxel,seventy - two hours later,cell viability was detected using MTT as-say. MDA - MB - 231 - Cherry and MDA - MB - 231 - miR - 374 cells were treated with docetaxel at a final concen-tration of 3. 2 nmol/ L,and colony - forming assay was used to detect the cell Proliferation activity. The cell aPoPtosis rate and cell cycle were detected by flow cytometry. Results:The relative exPression of miR - 374 in MDA - MB -231 - miR - 374 was significantly higher than that in MDA - MB - 231 - Cherry cells. ComPared with control cells, the Proliferation and colony - forming abilities of MDA - MB - 231 - miR - 374 cells imProves significantly,the Per-centages of cell aPoPtosis decreased,the cells at G0 / G1 Phase decreased,and those at S and G2 / M Phases increased, and the aPoPtosis rate of cells decreased significantly. Conclusion:The overexPression of miR - 374 may significantly enhance breast cancer MDA - MB - 231cells to docetaxel resistance.