CAJ | 학술논문

混合模式毛细管整体色谱柱由于保留机理多样，具有很好的应用前景。本文以[2-（甲基丙烯酰基氧基）乙基]二甲基-（3-磺酸丙基）氢氧化铵（ SPE ）为单体，乙二醇二甲基丙烯酸酯（ EDMA ）为交联剂，偶氮二异丁腈（ AIBN）为引发剂，正丙醇/1，4-丁二醇/水三元体系为致孔剂，制备了聚合物基质 SPE-co-EDMA 毛细管液相色谱整体柱。通过系统优化致孔剂和反应物种类和配比、引发剂的用量、反应时间和反应温度等因素，提高了整体柱的柱效、机械强度、渗透性和重复性。结果表明该毛细管整体柱在10 MPa 内具有良好的机械强度，渗透性为2.17×10-14 m2，而且批次内和批次间峰面积的重现性（RSD）分别为1.0％和4.6％。以极性和非极性的多种化合物评价了该毛细管整体柱的色谱性能，结果表明该柱在高有机相中具有亲水相互作用机理，在低有机相中具有反相作用机理，显示出混合模式分离特性。
혼합모식모세관정체색보주유우보류궤리다양，구유흔호적응용전경。본문이[2-（갑기병희선기양기）을기]이갑기-（3-광산병기）경양화안（ SPE ）위단체，을이순이갑기병희산지（ EDMA ）위교련제，우담이이정정（ AIBN）위인발제，정병순/1，4-정이순/수삼원체계위치공제，제비료취합물기질 SPE-co-EDMA 모세관액상색보정체주。통과계통우화치공제화반응물충류화배비、인발제적용량、반응시간화반응온도등인소，제고료정체주적주효、궤계강도、삼투성화중복성。결과표명해모세관정체주재10 MPa 내구유량호적궤계강도，삼투성위2.17×10-14 m2，이차비차내화비차간봉면적적중현성（RSD）분별위1.0％화4.6％。이겁성화비겁성적다충화합물평개료해모세관정체주적색보성능，결과표명해주재고유궤상중구유친수상호작용궤리，재저유궤상중구유반상작용궤리，현시출혼합모식분리특성。
Based on the diversity of the retention mechanism ,mixed-mode capillary monolithic columns have broad application prospect. In this work,a novel capillary polymer monolithic column was prepared with [ 2-( methacryloyloxy )ethyl ]dimethyl-( 3-sulfopropyl )ammonium hydroxide( SPE)as monomer,ethylene dimethacrylate( EDMA)as crosslinking agent,azobi-sisobutyronitrile( AIBN ) as initiator and butylalcohol/1,4-butanediol/water as ternary poro-gens. Under the optimized reaction conditions including the proportion of monomer and poro-gens,amount of initiator,reaction temperature and polymerization time,the monolithic col-umn showed good mechanical strength up to 10 MPa,high permeability of 2. 17 × 10-14 m 2 and good repeatability. The peak area RSDs of column-to-column and batch-to-batch reproducibility were 1. 0% and 4. 6%,respectively. Finally,the capillary monolithic column was evaluated with polar and non-polar test mixtures. It showed hydrophilic interaction under high organic phase while hydrophobic interaction under low organic phase,indicating it a mixed-mode monolithic column.