CAJ | 학술논문

目的观察复方酮替芬滴眼剂(含0.1%酮替芬及0.0005%地塞米松)对实验大鼠急性过敏性结膜炎的治疗作用. 方法雄性Wistar大鼠27只随机分为3组(复方酮替芬、0.1%酮替芬及地塞米松组),每组9只,各预先腹腔注射卵清蛋白致敏液(100μg卵清蛋白及20mg硫酸铝钾溶于1ml磷酸盐缓冲液,pH7.4),两周后以10%卵清蛋白液(溶于磷酸盐缓冲液)10μl滴眼攻击,引起结膜速发型过敏反应.攻击前15min以1mol.l-1DL-二巯苏糖醇20μl作眼部预处理,临攻击时静脉注射Evans蓝(EB)溶液(约2mg/100g);攻击前60,45,30,15min及攻击后15,30min各组1眼给药,对侧眼为溶媒.攻击后1h处死所有动物,测定各眼EB渗出量,进行比较,计算药物抑制率. 结果各组药物治疗眼EB外渗量均低于溶媒治疗眼,复方酮替芬组和0.1%酮替芬组显示统计学有显著意义.复方酮替芬抑制率为53.98%±15.57%,显著高于0.1%酮替芬(27.91%±28.36%,P＝0.028)及0.0005%地塞米松(11.90%±34.16%,P＝0.004). 结论结果提示复方酮替芬滴眼剂具有良好的抗过敏作用.
목적관찰복방동체분적안제(함0.1%동체분급0.0005%지새미송)대실험대서급성과민성결막염적치료작용. 방법웅성Wistar대서27지수궤분위3조(복방동체분、0.1%동체분급지새미송조),매조9지,각예선복강주사란청단백치민액(100μg란청단백급20mg류산려갑용우1ml린산염완충액,pH7.4),량주후이10%란청단백액(용우린산염완충액)10μl적안공격,인기결막속발형과민반응.공격전15min이1mol.l-1DL-이구소당순20μl작안부예처리,림공격시정맥주사Evans람(EB)용액(약2mg/100g);공격전60,45,30,15min급공격후15,30min각조1안급약,대측안위용매.공격후1h처사소유동물,측정각안EB삼출량,진행비교,계산약물억제솔. 결과각조약물치료안EB외삼량균저우용매치료안,복방동체분조화0.1%동체분조현시통계학유현저의의.복방동체분억제솔위53.98%±15.57%,현저고우0.1%동체분(27.91%±28.36%,P＝0.028)급0.0005%지새미송(11.90%±34.16%,P＝0.004). 결론결과제시복방동체분적안제구유량호적항과민작용.
Objective:To examine the effect of compound ketotifen eye drops(CKF,containing 0.1% ketotifen and 0.000 5% dexamethasone) on experimental acute allergic conjunctivitis in rats.Methods:27 male Wistar rats were immunized by intraperitoneal injection of egg albumin and were randomized into three groups: CKF,0.1% ketotifen (KF) and 0.000 5% dexamethasone (DM) Rats in each group received the respective eye drops in one eye and vehicle in the other eye 60,45,30,15 min before and 15,30 min after challenge.Immediately before challenge,the rats were injected intravenously with Evans Blue(EB).The challenge was performed by topical instillation of egg albumin,and 60 min later,the animals were killed and the dye was extracted from the eyes.The intensity of EB extravasation was determined by spectrophotometry at 620nm and inhibitory rates were calculated.Results:CKF,KF and DM suppressed EB extravasation significantly.The inhibitory rate was 53.98%±15.57% for CKF,27.91%±28.36% for KF and 11.90%±34.16% for DM.CKF was more effective than both KF and DM on inhibiting dye vascular leakage in the eyes (P＝0.028,0.004,respectively).Conclusion:These data clearly indicate that the compound of ketotifen eye drops has potential as a topical ocular formulation for anti-allergic disease.