CAJ | 학술논문

以提高大肠杆菌耐热性为目的，基于腾冲嗜热菌（Thermoanaerobacter tengcongensis MB4）热激蛋白基因T.te-HSP20构建了诱导型耐热元器件 T7-T.te-HSP20和组成型耐热元器件 gapA-T.te-HSP20，转入大肠杆菌（Escherichia coli）获得工程菌 E. coli-TH 和 E. coli-GH。工程菌E. coli-TH在30℃和IPTG诱导下，目标蛋白呈可溶性表达，经50℃热激30 min后，存活率提高了3.2倍。高温发酵表明gapA-T.te-HSP20扩宽了工程菌E. coli-GH的最适生长温度的范围（37～43℃），较大程度提高了大肠杆菌的耐热性。抗逆性分析还发现工程菌E. coli-GH具备了耐热与耐丁醇的双重功能，并有一定的抗乙酸和乙醇能力。为工业梯度升温发酵生产生物基产品的高效制造、节省成本提供了新思路。
이제고대장간균내열성위목적，기우등충기열균（Thermoanaerobacter tengcongensis MB4）열격단백기인T.te-HSP20구건료유도형내열원기건 T7-T.te-HSP20화조성형내열원기건 gapA-T.te-HSP20，전입대장간균（Escherichia coli）획득공정균 E. coli-TH 화 E. coli-GH。공정균E. coli-TH재30℃화IPTG유도하，목표단백정가용성표체，경50℃열격30 min후，존활솔제고료3.2배。고온발효표명gapA-T.te-HSP20확관료공정균E. coli-GH적최괄생장온도적범위（37～43℃），교대정도제고료대장간균적내열성。항역성분석환발현공정균E. coli-GH구비료내열여내정순적쌍중공능，병유일정적항을산화을순능력。위공업제도승온발효생산생물기산품적고효제조、절성성본제공료신사로。
To improve the heat resistance of Escherichia coli, an inducible heat-resistance device T7-T.te-HSP20 and a constitutive heat-resistance device gapA-T.te-HSP20 based on T.te-HSP20 gene from Thermoanaerobacter tengcongensis MB4, and corresponding engineered strains E. coli-TH and E. coli-GH were constructed. The targeted protein was expressed in solubility after IPTG induction at 30℃ in E. coli-TH. Meanwhile, the survival rate of E. coli-TH was 3.2 times higher than the control at 50℃ for 30 min. The result of high-temperature fermentation showed that the optimum temperature range of E. coli-GH was broadened (37-43℃) under the regulation of heat resistance device gapA-T.te-HSP20. Stress resistance analysis showed that E. coli-GH not only possessed heat resistance and butanol resistance, but also had some resistance to acetic acid and ethanol. These results provide a new idea for modern microorganisms industry.