中国园艺文摘
中國園藝文摘
중국완예문적
CHINESE HORTICULTURE ABSTRACTS
2014年
6期
10-12
,共3页
紫秆海芋%芽%组织培养
紫稈海芋%芽%組織培養
자간해우%아%조직배양
Alocasia macrorrhiza%bud%Tissue culture
用植物组织培养方法建立紫秆海芋快繁体系，结果表明：紫秆海芋外植体诱导成活最好的部位是芽，外植体诱导愈伤组织最佳培养配方是：MS+BA 1.5 mg/L+NAA 1.5 mg/L+碳粉3 g/L，诱导成活率高达100%，并且无污染；愈伤组织诱导成苗的最佳配方是：MS+TDZ 2.0 mg/L+NAA 1.0 mg/L，诱导成苗率为100%；继代增殖的最佳配方是：MS+BA 1.5 mg/L+KT 1.0 mg/L+NAA 0.5 mg/L+琼脂5.8 g/L，增殖率410%，并且植株长势良好；生根培养的最佳配方是：MS+BA 0.2 mg/L+NAA 1.0 mg/L+IBA 1.0 mg/L，并且平均根数、平均根长、平均根粗3个数值较理想。
用植物組織培養方法建立紫稈海芋快繁體繫，結果錶明：紫稈海芋外植體誘導成活最好的部位是芽，外植體誘導愈傷組織最佳培養配方是：MS+BA 1.5 mg/L+NAA 1.5 mg/L+碳粉3 g/L，誘導成活率高達100%，併且無汙染；愈傷組織誘導成苗的最佳配方是：MS+TDZ 2.0 mg/L+NAA 1.0 mg/L，誘導成苗率為100%；繼代增殖的最佳配方是：MS+BA 1.5 mg/L+KT 1.0 mg/L+NAA 0.5 mg/L+瓊脂5.8 g/L，增殖率410%，併且植株長勢良好；生根培養的最佳配方是：MS+BA 0.2 mg/L+NAA 1.0 mg/L+IBA 1.0 mg/L，併且平均根數、平均根長、平均根粗3箇數值較理想。
용식물조직배양방법건립자간해우쾌번체계，결과표명：자간해우외식체유도성활최호적부위시아，외식체유도유상조직최가배양배방시：MS+BA 1.5 mg/L+NAA 1.5 mg/L+탄분3 g/L，유도성활솔고체100%，병차무오염；유상조직유도성묘적최가배방시：MS+TDZ 2.0 mg/L+NAA 1.0 mg/L，유도성묘솔위100%；계대증식적최가배방시：MS+BA 1.5 mg/L+KT 1.0 mg/L+NAA 0.5 mg/L+경지5.8 g/L，증식솔410%，병차식주장세량호；생근배양적최가배방시：MS+BA 0.2 mg/L+NAA 1.0 mg/L+IBA 1.0 mg/L，병차평균근수、평균근장、평균근조3개수치교이상。
Rapid propagation system was established based on the culture techniquein vitro. Results were showed as follows. The explant which was most likely to survivein vitro was the bud. The callus were induced using the medium: MS+BA 1.5 mg/L+NAA 1.5 mg /L+carbon powder 3 g/L, and the induction survival rate was up to 100% without contamination. The best formulations to induce the callus into seedlings were MS+TDZ 2.0 mg/L+NAA 1.0mg/L, and the seedling rate was 100%. The subculture micropropagation’s formula with 410% reproduction rate was MS+BA 1.5 mg/L+KT 1.0 mg/L+NAA 0.5 mg/L+ agar 5.8 g/L. The composition of rooting culture was MS+BA 0.2 mg/L+NAA 1.0 mg/L+IBA 1.0 mg/L, obtaining ideal average root numbers, length, width.
