CAJ | 학술논문

背景与目的半乳凝素-3（Galectin-3）参与许多生理和病理过程，与肿瘤的发生、发展、转移关系密切。本研究旨在探讨Galectin-3在非小细胞肺癌（non-small cell lung cancer, NSCLC）中的表达情况及其与临床病理特征间的关系，探索其在NSCLC发生、发展中的作用。方法采用S-P免疫组化方法检测62例NSCLC组织和17例正常肺组织中Galectin-3的表达情况，并用RT-PCR方法在转录水平进行验证，并结合相关的临床病理资料进行分析。结果免疫组化结果显示Galectin-3在83.8%（52/62）的NSCLC组织中阳性表达。NSCLC组织中Galectin-3表达阳性率明显高于正常肺组织（χ2=7.936, P<0.01），其在癌组织中的表达与患者年龄、性别和病理分型无关。Galectin-3与肿瘤的分化程度（χ2=8.577, P<0.05）和临床分期（χ2=5.287, P<0.05）相关，其中与分化程度（r=-0.292, P<0.05）的表达呈负相关，与临床分期（r=0.336, P<0.05）的表达呈正相关。RT-PCR结果显示，Galectin-3在NSCLC组织中的表达明显高于正常肺组织，随访结果表明Galectin-3高表达者总生存小于低表达者（P=0.045)。结论 Galectin-3可能是肺癌诊断的重要辅助基因，Galectin-3有望与肺癌其它标志物联合检查以提高诊断率，提示患者预后。
배경여목적반유응소-3（Galectin-3）삼여허다생리화병리과정，여종류적발생、발전、전이관계밀절。본연구지재탐토Galectin-3재비소세포폐암（non-small cell lung cancer, NSCLC）중적표체정황급기여림상병리특정간적관계，탐색기재NSCLC발생、발전중적작용。방법채용S-P면역조화방법검측62례NSCLC조직화17례정상폐조직중Galectin-3적표체정황，병용RT-PCR방법재전록수평진행험증，병결합상관적림상병리자료진행분석。결과면역조화결과현시Galectin-3재83.8%（52/62）적NSCLC조직중양성표체。NSCLC조직중Galectin-3표체양성솔명현고우정상폐조직（χ2=7.936, P<0.01），기재암조직중적표체여환자년령、성별화병리분형무관。Galectin-3여종류적분화정도（χ2=8.577, P<0.05）화림상분기（χ2=5.287, P<0.05）상관，기중여분화정도（r=-0.292, P<0.05）적표체정부상관，여림상분기（r=0.336, P<0.05）적표체정정상관。RT-PCR결과현시，Galectin-3재NSCLC조직중적표체명현고우정상폐조직，수방결과표명Galectin-3고표체자총생존소우저표체자（P=0.045)。결론 Galectin-3가능시폐암진단적중요보조기인，Galectin-3유망여폐암기타표지물연합검사이제고진단솔，제시환자예후。
Background and objective Galectin-3 is involved in many physical and pathologic process, and is closely correlated with tumorigenesis, development and metastasis. hTe aim of this study is to investigate the gene expression and signiifcance of Galectin-3 in normal lung tissue and non-small cell lung cancer (NSCLC) tissue. Methods Gene expres-sion was examined using the immunohistochemical S-P method in 62 NSCLC cases and in 17 cases with normal lung tissue. RT-PCR veriifed the expression of Galectin-3 at the transcriptional level. Results hTe expression rate of Galectin-3 in NSCLC tissue was 83.8%(52/62), which was signiifcantly higher than that in normal lung tissue (P<0.01). Galectin-3 expression was not correlated with sex (χ2=0.113, P>0.05), age (χ2=0.220, P>0.05), and histological type (χ2=0.012, P>0.05), but was nega-tively correlated with pathological differentiation (r=-0.292, P<0.05) and positively correlated with the clinical stage (r=0.336, P<0.05) in NSCLC tissue. RT-PCR results showed that the expression level of Galectin-3 in NSCLC tissue was signiifcantly higher than in normal lung tissue. Conclusion Galectin-3 may be useful in lung cancer diagnosis and may increase diagnosis rate when combined with other lung cancer markers.