CAJ | 학술논문

背景与目的肺癌脑转移是临床常见的严重并发症，由于脑部结构和功能的特殊性、脑转移检测方法的局限性，预后很差。本研究旨在筛选人肺腺癌脑转移细胞株CPA-Yang1-BR以及建立裸小鼠动物模型和检测方法。方法将人肺腺癌细胞株CPA-Yang1-GFP接种于裸小鼠左心室，约7周-8周后比较三种小动物显像方法：micro PET/CT显像，X线、放射性核素、荧光（三合一）活体成像系统和小动物线圈MRI显像，实验证明MRI显像是最准确的小鼠脑转移病灶检测方法。脑核磁共振成像（magnatic resonance imaging, MRI）显像找到脑转移灶，深麻醉处死裸小鼠取出脑转移病灶，部分病理验证，部分行原代培养后获得人肺腺癌脑转移细胞，再次接种裸小鼠，用上述方法重复以上体内外循环4次，观察脑转移形成情况。结果获得人肺腺癌脑转移细胞株CPA-Yang1-BR及其裸小鼠模型。结论 CPA-Yang1细胞经反复裸小鼠脑组织内外筛选的方法可获得具有高转移潜能的裸小鼠脑转移模型，为肺癌脑转移的生物学研究提供了一个良好的技术平台。小动物线圈MRI或micro MRI活体显像是检测小鼠肺癌脑转移敏感、准确、无创伤的显像方法。
배경여목적폐암뇌전이시림상상견적엄중병발증，유우뇌부결구화공능적특수성、뇌전이검측방법적국한성，예후흔차。본연구지재사선인폐선암뇌전이세포주CPA-Yang1-BR이급건립라소서동물모형화검측방법。방법장인폐선암세포주CPA-Yang1-GFP접충우라소서좌심실，약7주-8주후비교삼충소동물현상방법：micro PET/CT현상，X선、방사성핵소、형광（삼합일）활체성상계통화소동물선권MRI현상，실험증명MRI현상시최준학적소서뇌전이병조검측방법。뇌핵자공진성상（magnatic resonance imaging, MRI）현상조도뇌전이조，심마취처사라소서취출뇌전이병조，부분병리험증，부분행원대배양후획득인폐선암뇌전이세포，재차접충라소서，용상술방법중복이상체내외순배4차，관찰뇌전이형성정황。결과획득인폐선암뇌전이세포주CPA-Yang1-BR급기라소서모형。결론 CPA-Yang1세포경반복라소서뇌조직내외사선적방법가획득구유고전이잠능적라소서뇌전이모형，위폐암뇌전이적생물학연구제공료일개량호적기술평태。소동물선권MRI혹micro MRI활체현상시검측소서폐암뇌전이민감、준학、무창상적현상방법。
Background and objective Lung cancer is the leading cause of cancer-related death in men and wom-en. It is also the most common cause of brain metastases. A brain metastasis model is diffcult to be established because of the presence of the blood-brain barrier (BBB) and the lack of optimal methods for detecting brain metastasis in nude mice. hTus, the establishment of a Chinese lung adenocarcinoma cell line and its animal model with brain metastasis potency and in vivo research is of great signiifcance. Methods CPA-Yang1 cells were obtained from a patient with human lung adeno-carcinoma by lentiviral vector-mediated transfection of green lfuorescence protein. Intracardiac inoculation of the cells was performed in nude mice, and brain metastatic lesions were detected using micro 18F FDG-PET/CT scanners, small animal in vivo imaging system for lfuorescence, radionuclide and X ray fused imaging, magnetic resonance imaging (MRI) with sense body detection, and resection. hTe samples were divided into two parts for cell culture and histological diagnosis. hTe process was repeated in vivo and in vitro for four cycles to obtain a novel cell clone, CPA-Yang1-BR. Results A novel cell clone, CPA-Yang1-BR, was obtained with a brain metastatic rate of 50%. hTe use of MRI for the detection of brain metasta-ses has obvious advantages. Conclusion An experimental Chinese lung adenocarcinoma cell clone (CPA-Yang1-BR) and its animal model with brain metastasis potency in nude mice were established. MRI with sense body or micro MRI may be used as a sensitive, accurate, and noninvasive method to detect experimental brain metastases in intact live immunodeifcient mice. hTe results of this study may serve as a technical platform for brain metastases from lung adenocarcinoma.