医学美学美容（中旬刊）
醫學美學美容（中旬刊）
의학미학미용（중순간）
MEDICAL AESTHETICS AND COSMETOLOGY
2013年
8期
12-13,14
,共3页
耿红芳%何龙%孟凡民(通讯作者)
耿紅芳%何龍%孟凡民(通訊作者)
경홍방%하룡%맹범민(통신작자)
硫化氢%炎症反应
硫化氫%炎癥反應
류화경%염증반응
Hydrogen sulfide%Inflammatory response
目的探讨外源性硫化氢（H2S）供体硫氢化钠（NaHS）对部分肝切除小鼠术后炎症反应影响。方法成年雄性昆明小鼠72只，随机分为3组（n＝24）：对照组（NS组）、手术组（Surgery 组）和硫氢化钠＋手术组（NaHS＋ surgery 组）。术后第1、3、7天分别从各组中随机抽取8只动物，麻醉下经眼眶采血，ELISA法检测外周血 TNF -α、IL -6浓度。结果与 NS组比较，Surgery 组及 NaHS＋ Surgery 组术后血清 TNF -α、IL -6浓度高于NS组（p＜0．05）；与Surgery组比较，NaHS＋ surgery组术后血清 TNF -α、IL -6浓度明显低于Surgery组（p＜0．05）。结论腹腔注射 NaHS能够减轻肝切除小鼠术后早期的外周炎症反应。
目的探討外源性硫化氫（H2S）供體硫氫化鈉（NaHS）對部分肝切除小鼠術後炎癥反應影響。方法成年雄性昆明小鼠72隻，隨機分為3組（n＝24）：對照組（NS組）、手術組（Surgery 組）和硫氫化鈉＋手術組（NaHS＋ surgery 組）。術後第1、3、7天分彆從各組中隨機抽取8隻動物，痳醉下經眼眶採血，ELISA法檢測外週血 TNF -α、IL -6濃度。結果與 NS組比較，Surgery 組及 NaHS＋ Surgery 組術後血清 TNF -α、IL -6濃度高于NS組（p＜0．05）；與Surgery組比較，NaHS＋ surgery組術後血清 TNF -α、IL -6濃度明顯低于Surgery組（p＜0．05）。結論腹腔註射 NaHS能夠減輕肝切除小鼠術後早期的外週炎癥反應。
목적탐토외원성류화경（H2S）공체류경화납（NaHS）대부분간절제소서술후염증반응영향。방법성년웅성곤명소서72지，수궤분위3조（n＝24）：대조조（NS조）、수술조（Surgery 조）화류경화납＋수술조（NaHS＋ surgery 조）。술후제1、3、7천분별종각조중수궤추취8지동물，마취하경안광채혈，ELISA법검측외주혈 TNF -α、IL -6농도。결과여 NS조비교，Surgery 조급 NaHS＋ Surgery 조술후혈청 TNF -α、IL -6농도고우NS조（p＜0．05）；여Surgery조비교，NaHS＋ surgery조술후혈청 TNF -α、IL -6농도명현저우Surgery조（p＜0．05）。결론복강주사 NaHS능구감경간절제소서술후조기적외주염증반응。
Objective The present study was designed to investigate effect of sodium hydrosulfide (NaHS) ,a H2S exogenous donor ,on hepatolobectomy triggers inflammatory response in adult mice and the possible mechanisms .Methods :Seventy two Kunming male mice were randomly assigned to three groups :1) Control group (group NS ;n=24) .2) Surgery group (group Surgery ;n=24) .3) Sodium hydrosul-fide plus Surgery group (group NaHS+ surgery ;n=24) .On post -surgical 1 ,3 ,7 mice were anesthetized and blood sample were obtained via orbital venous plexus .TNF -α and IL -6 were measured by ELISA .The mice after the blood samples collected were euthanized ,and hippocampus were removed .The expressions of TNF -α ,IL -1β ,and IL -6 protein in hippocampus were determined by Western blot .Re-sults :Serum levels of TNF -αand IL -6 were not significantly different in group NaHS when compared with group NS after surgery ,re-spectively(p>0 .05) ,but group Surgery and group NaHS+ surgery Serum TNF -αand IL -6 were significantly different in group Surgery after surgery(p<0 .05) ,Compared with group Surgery ,Serum IL -6 were significantly different in group NaHS + surgery after surgery(p<0 .05) .Conclusion :Pre- and after surgery treatment with NaHS significantly attenuated hepatolobectomy triggers be able to improve the peripheral inflammatory response .
