CAJ | 학술논문

本研究采用分光光度法及琼脂糖凝胶电泳比较从黑熊组织、血液以及毛发中提取的黑熊基因组DNA，发现提取的DNA浓度及纯度均可满足后续试验的需要。其中毛发DNA提取方法具有高灵敏度、操作简单、非损伤等特点，有助于开展珍稀濒危物种和性情凶猛动物的遗传特性研究。本文还根据文献中黑熊的11个微卫星位点设计并合成引物，进行了不同引物PCR最佳反应条件的筛选。对黑熊DNA基因组PCR扩增结果发现其中10对引物能扩增出较明显的扩增带，为后续的黑熊亲子鉴定及遗传特异性分析实验提供了有价值的资料，为其亲权概率的准确性提供了良好的参考依据。
본연구채용분광광도법급경지당응효전영비교종흑웅조직、혈액이급모발중제취적흑웅기인조DNA，발현제취적DNA농도급순도균가만족후속시험적수요。기중모발DNA제취방법구유고령민도、조작간단、비손상등특점，유조우개전진희빈위물충화성정흉맹동물적유전특성연구。본문환근거문헌중흑웅적11개미위성위점설계병합성인물，진행료불동인물PCR최가반응조건적사선。대흑웅DNA기인조PCR확증결과발현기중10대인물능확증출교명현적확증대，위후속적흑웅친자감정급유전특이성분석실험제공료유개치적자료，위기친권개솔적준학성제공료량호적삼고의거。
In this study,we compared the quality of genome DNA extracted from the tissue or blood or hair of black bears using spectrophotgmeter and agarose gel eleetrophoresis and found that the concentration and purity of genome DNA we obtained can all meet the needs of the subsequent experiments. Among three resources of genomic DNA, the hair extraction method has many benefits like high sensitivity, simple operation, non-damage etc, may solve the difficult samplings from the rare and endangered animal species or the temperament fierce animals, and may help the investigators to conduct many important genomic studies of them. We investigated 11 microsatellite loci of the black bear,designed and synthesized every set of primers depending on the sequences of each microsatellite, finally screened the best reaction conditions for polymerase chain reaction (PCR) of every microsatellite. The PCR results showed ~hat 10 in 11 microsatellites mentioned above have displayed relatively clear PCR bands using synthesized primer set respectively. These 10 microsatellites have high values in developing accurate paternity test in the black bear species, and will be a good reference basis to the paternity rates calculations.