CAJ | 학술논문

以1~18月龄乌珠穆沁羊为研究对象，对其骨骼肌内结缔组织中Ⅰ型胶原蛋白进行提取，并进行纯度鉴定；利用高效液相色谱测定所提取的Ⅰ型胶原蛋白中3种主要氨基酸含量，并依据羟脯氨酸含量计算出Ⅰ型胶原蛋白的含量。结果表明，随着月龄的增加，乌珠穆沁羊骨骼肌内结缔组织中羟脯氨酸、甘氨酸和脯氨酸含量均有缓慢增加的趋势；Ⅰ型胶原蛋白的含量随着月龄的增加而增加（p<0.05）。解析乌珠穆沁羊生长过程中肌内结缔组织中Ⅰ型胶原蛋白含量的变化规律。对进一步研究家畜生长过程中肌内结缔组织中胶原蛋白的性质及其对肉品质的影响起着重要的作用。
이1~18월령오주목심양위연구대상，대기골격기내결체조직중Ⅰ형효원단백진행제취，병진행순도감정；이용고효액상색보측정소제취적Ⅰ형효원단백중3충주요안기산함량，병의거간포안산함량계산출Ⅰ형효원단백적함량。결과표명，수착월령적증가，오주목심양골격기내결체조직중간포안산、감안산화포안산함량균유완만증가적추세；Ⅰ형효원단백적함량수착월령적증가이증가（p<0.05）。해석오주목심양생장과정중기내결체조직중Ⅰ형효원단백함량적변화규률。대진일보연구가축생장과정중기내결체조직중효원단백적성질급기대육품질적영향기착중요적작용。
Ujumqin sheep, aged from 1 to 18 months, are employed in this study. The type I collagen of intramuscular connective tissues is extracted and the three major amino acids by high performance liquid chromatographic (HPLC) are determined, and then the content of typeⅠcollagen is calculated based on the hydroxyproline. The results show that: the three amino acids contents of intramuscular connective tissues are increased, and the content of typeⅠcollagen is increased with growth in gradually (p<0.05) . The changes of type I collagen in the intramuscular connective tissue during the growth of Ujumqin sheep are analyzed. It is play an important role of further study on the characteristic of intramuscular collagen and the effects of collagen on the meat quality and property during the growth of livestock.