CAJ | 학술논문

　　本研究建立了动物尿液中9种β-受体激动剂（苯乙醇胺A、沙丁胺醇、特布他林、莱克多巴胺、克伦特罗、溴布特罗、马布特罗、西布特罗、喷布特罗）的液相色谱-串联质谱法（UPLC- MS/MS）。样品经醋酸铵为提取溶剂，固相萃取柱净化，用UPLC- MS/MS检测和确证，内标法定量。质谱分析采用电喷雾电离、正离子扫描、多反应监测模式。试验结果表明，9种兴奋剂在0.5～100μg/L范围内具有良好的线性，相关系数在0.995～0.999，检测限为0.1μg/L，定量限为0.2μg/L，9种兴奋剂的方法平均回收率为60.63%～94.12%，相对标准偏差在1.26%～14.74%。试验结果表明，该方法提取效果好，具有良好的灵敏度、回收率和重复性，能满足动物尿液中兴奋剂等兽药残留分析要求。
　　본연구건립료동물뇨액중9충β-수체격동제（분을순알A、사정알순、특포타림、래극다파알、극륜특라、추포특라、마포특라、서포특라、분포특라）적액상색보-천련질보법（UPLC- MS/MS）。양품경작산안위제취용제，고상췌취주정화，용UPLC- MS/MS검측화학증，내표법정량。질보분석채용전분무전리、정리자소묘、다반응감측모식。시험결과표명，9충흥강제재0.5～100μg/L범위내구유량호적선성，상관계수재0.995～0.999，검측한위0.1μg/L，정량한위0.2μg/L，9충흥강제적방법평균회수솔위60.63%～94.12%，상대표준편차재1.26%～14.74%。시험결과표명，해방법제취효과호，구유량호적령민도、회수솔화중복성，능만족동물뇨액중흥강제등수약잔류분석요구。
A method of ultra performance liquid chromatography-tandem mass spec-trometry (UPLC-MS/MS)for simultaneous determination of nine β-receptor agonists, such as phenylethanolamineA,salbutamol,terbutaline,ractopamine,clenbuterol,bromb-uterol,mabuterol,cimbut-erol and penbutolol in animal urine was developed.The β-re-ceptor agonists was extracted with ammonium acetate,then cleaned up with a mcx sol-id-phase extraction(SPE)column.The eluate was evaporate to driness with nitrogen. The limits of detection were 0.1 μg/L for all β-receptor agonists and the limits of quantification were 0.2 μg/L,the average recoveryies were 60.63%~94.12%.The rel-ative standard deviations were 1.26%~14.74%,the real sample tests showed this meth-od can be used for the sensitive and accurate determination of multi-β-receptor ag-onists residues in animal urine.