CAJ | 학술논문

目的：建立高效液相色谱法同时测定人血浆中异烟肼、乙酰异烟肼和吡嗪酰胺的浓度。方法：色谱柱为Inertsil ODS-SP（4.6 mm×150 mm，5μm），以甲醇∶0.05 mol·L-1磷酸二氢钾（三乙胺调pH 5.6）为流动相，柱温：40℃，流速：1.0 mL·min-1，进样体积：20μL，检测波长：268 nm。结果：3组分在线性范围内与峰面积线性关系良好（r2>0.9940），日内、日间精密度RSD<10%，平均回收率在80%~120%。结论：本方法专属性好，样品处理简单，符合生物样品分析要求。
목적：건립고효액상색보법동시측정인혈장중이연정、을선이연정화필진선알적농도。방법：색보주위Inertsil ODS-SP（4.6 mm×150 mm，5μm），이갑순∶0.05 mol·L-1린산이경갑（삼을알조pH 5.6）위류동상，주온：40℃，류속：1.0 mL·min-1，진양체적：20μL，검측파장：268 nm。결과：3조분재선성범위내여봉면적선성관계량호（r2>0.9940），일내、일간정밀도RSD<10%，평균회수솔재80%~120%。결론：본방법전속성호，양품처리간단，부합생물양품분석요구。
Objective: To determine the concentrations of isoniazid, acetylisoniazid and pyrazinamide in human plasma by RP-HPLC. Methods: The HPLC method was carried out on an Inertsil ODS-SP (4.6 × 150 mm, 5μm) column with 0.05 mol·L-1 KH2PO4 (adjusting PH to 6.0 with triethylamine)-methanol as mo-bile phase. The column temperature was 40℃, the flow rate was 1.0 mL·min-1 and the detection wave-length was 268 nm. Results: All calibration curves had good linearity (r2>0.99) within their tested ranges. The intra- and inter-day precision was within 10%, the extraction recovery was between 80% and 120%. Conclusion: The method is sensitive, simple and rapid. It is suitable for analyzing the drugs in biological samples.