CAJ | 학술논문

目的：建立丹路口服液质量标准。方法：采用TLC法对丹路口服液中丹参、当归、川芎、红花进行定性鉴别；以HPLC法对其赤芍中的芍药苷进行含量测定。色谱柱：Symmetry C18柱（200 mm×4.6 mm，5μm）；流动相：乙腈-1‰磷酸水溶液（19∶81）；流速：1.0 mL·min-1；检测波长：230 nm；柱温：30℃。结果：在TLC色谱中检出丹参、当归、川芎、红花；芍药苷进样量在6.14-61.40μg mL-1范围内线性关系良好，r=0.9998；平均加样回收率101.0％，RSD%为2.5％。结论：所建立的方法简便可行，重现性好，为丹路口服液质量控制提供了方法。
목적：건립단로구복액질량표준。방법：채용TLC법대단로구복액중단삼、당귀、천궁、홍화진행정성감별；이HPLC법대기적작중적작약감진행함량측정。색보주：Symmetry C18주（200 mm×4.6 mm，5μm）；류동상：을정-1‰린산수용액（19∶81）；류속：1.0 mL·min-1；검측파장：230 nm；주온：30℃。결과：재TLC색보중검출단삼、당귀、천궁、홍화；작약감진양량재6.14-61.40μg mL-1범위내선성관계량호，r=0.9998；평균가양회수솔101.0％，RSD%위2.5％。결론：소건립적방법간편가행，중현성호，위단로구복액질량공제제공료방법。
Objective: To establish a quality standard for Danlu Oral Liquid. Methods: Salvia mihiorrhiza Bge, Angelica sinensis(Oliv) Diels, Ligustici Chuanxiong Hort, Carthamus tinctorius L were indentified by TLC. HPLC was applied to deter-mine the Paeoniflorin;The separation was performed on a Symmetry of C18 column(200 mm×4.6 nnn, 5μm); The mobile phase was consisted of acetonitrile and water(1‰ phosphoric acid)(19∶81); The detection wavelength was 230 nm; Flow rate was 1.0 mL·min-1; Column temperature was 30℃. Results: Salvia miltiorrhiza Bge, Angelica sinensis(Oliv) Diels, Ligusticum chuanxiong Hort, Carthamus tinctorius L could be detected by TLC. Paeoniflorin showed a good linear relationship at 6.14-61.40 μg·mL-1, r=0.9998; The average recovery of the added index was 101.0%, and RSD% was 2.5%. Conclusion: The method is simple, feasible and reproducible. The established quality standard can be used for the quality control of this medicine.