CAJ | 학술논문

以蓝狐（ Alopex lagopus）胆囊胆汁为实验材料，采用正交试验建立了测定狐胆汁中结合型胆汁酸成分的高效液相色谱分析法。结果表明，采用Hypersil BDS C18（4．6 mm×250 mm，5μm）为色谱柱，V（甲醇）∶V（30 mmol/L磷酸二氢钾）＝70∶30的溶液（pH＝3．8）为流动相进行等度洗脱，流速为1．0 mL/min，检测波长为205 nm，柱温为25℃。测得狐胆汁中含有牛磺胆酸和牛磺鹅去氧胆酸，它们的峰面积比分别为35．05％和9．23％，不含牛磺熊去氧胆酸。各组分在3．33～10 g/L范围内具有良好的线性关系。本方法具有简单快速、分离效果好、重复性好等优点，可以用来分析狐胆汁中结合型胆汁酸。
이람호（ Alopex lagopus）담낭담즙위실험재료，채용정교시험건립료측정호담즙중결합형담즙산성분적고효액상색보분석법。결과표명，채용Hypersil BDS C18（4．6 mm×250 mm，5μm）위색보주，V（갑순）∶V（30 mmol/L린산이경갑）＝70∶30적용액（pH＝3．8）위류동상진행등도세탈，류속위1．0 mL/min，검측파장위205 nm，주온위25℃。측득호담즙중함유우광담산화우광아거양담산，타문적봉면적비분별위35．05％화9．23％，불함우광웅거양담산。각조분재3．33～10 g/L범위내구유량호적선성관계。본방법구유간단쾌속、분리효과호、중복성호등우점，가이용래분석호담즙중결합형담즙산。
An experiment was conducted to build the method for compositing conjugated bile acid in fox bile by high performance liquid chromatography through orthogonal test with blue fox ( Alopex lagopus) gallbladder bile as experimental material. The column was Hypers-il BDS C18(4.6 mm×250 mm,5 μm).The solution of methanol/30 mmol/L potassium dihydrogen phosphate (70 ∶30, PH=3.8) was used as mobile phase in isocratic elution.Flow rate was 1.0 mL/min.The detector wavelength was 205 nm and the column temperature was 25℃.The fox bile contained taurocholic acid and taurochenode-oxycholic acid and their peak areas are 35.05%and 9.23%, respectively, without tauroursodeoxycholic acid.Each com-ponent has a good liner relationship in 3.33-10 g/L.The method is simple and rapid with accurate separation and good re-producibility.It can be used for analyzing conjugated bile acid in fox bile.