东北林业大学学报
東北林業大學學報
동북임업대학학보
JOURNAL OF NORTHEAST FORESTRY UNIVERSITY
2013年
8期
95-101
,共7页
严俊鑫%迟德富%张永强%宇佳%张晓娇
嚴俊鑫%遲德富%張永彊%宇佳%張曉嬌
엄준흠%지덕부%장영강%우가%장효교
水杨酸%白粉病%重瓣玫瑰%防御酶%次生代谢
水楊痠%白粉病%重瓣玫瑰%防禦酶%次生代謝
수양산%백분병%중판매괴%방어매%차생대사
Salicylic acid (SA)%Sphaerotheca pannosa (Wallr.)%R osa rugosa ‘Plena’%Protective enzyme%Sec-ondary metabolism
喷施不同浓度的水杨酸( SA),研究其引发重瓣玫瑰对白粉病的诱导抗性,实验结果表明,不同浓度SA诱导处理均对重瓣玫瑰白粉病有抑制效果,SA处理显著减低了重瓣玫瑰叶片白粉病病情指数,0.5 mmol/L SA诱导效果最好。不同浓度SA诱导(接菌)后,对重瓣玫瑰叶片苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶( POD)活性有显著影响,且对总酚质量分数、木质素相对值的影响明显。经0.5 mmol/L SA诱导后,叶片PPO活性、总酚质量分数均在诱导(接菌)后第7(5)天时达到最大值,分别是CK1(喷施清水)的1.55、1.71倍,是CK2(喷施清水+接种白粉菌)的1.33、1.50倍,且与CK1、CK2相比差异极显著( P<0.01)。1.0 mmol/L SA诱导(接菌)后PAL、POD活性在第7(5)天时达到最大值,分别是CK1的1.68、2.06倍,是CK2的1.35、1.56倍,且与CK1、CK2相比差异极显著( P<0.01)。0.5 mmol/L SA处理在整个试验期间诱导的木质素相对值均显著高于CK1、CK2。
噴施不同濃度的水楊痠( SA),研究其引髮重瓣玫瑰對白粉病的誘導抗性,實驗結果錶明,不同濃度SA誘導處理均對重瓣玫瑰白粉病有抑製效果,SA處理顯著減低瞭重瓣玫瑰葉片白粉病病情指數,0.5 mmol/L SA誘導效果最好。不同濃度SA誘導(接菌)後,對重瓣玫瑰葉片苯丙氨痠解氨酶(PAL)、多酚氧化酶(PPO)、過氧化物酶( POD)活性有顯著影響,且對總酚質量分數、木質素相對值的影響明顯。經0.5 mmol/L SA誘導後,葉片PPO活性、總酚質量分數均在誘導(接菌)後第7(5)天時達到最大值,分彆是CK1(噴施清水)的1.55、1.71倍,是CK2(噴施清水+接種白粉菌)的1.33、1.50倍,且與CK1、CK2相比差異極顯著( P<0.01)。1.0 mmol/L SA誘導(接菌)後PAL、POD活性在第7(5)天時達到最大值,分彆是CK1的1.68、2.06倍,是CK2的1.35、1.56倍,且與CK1、CK2相比差異極顯著( P<0.01)。0.5 mmol/L SA處理在整箇試驗期間誘導的木質素相對值均顯著高于CK1、CK2。
분시불동농도적수양산( SA),연구기인발중판매괴대백분병적유도항성,실험결과표명,불동농도SA유도처리균대중판매괴백분병유억제효과,SA처리현저감저료중판매괴협편백분병병정지수,0.5 mmol/L SA유도효과최호。불동농도SA유도(접균)후,대중판매괴협편분병안산해안매(PAL)、다분양화매(PPO)、과양화물매( POD)활성유현저영향,차대총분질량분수、목질소상대치적영향명현。경0.5 mmol/L SA유도후,협편PPO활성、총분질량분수균재유도(접균)후제7(5)천시체도최대치,분별시CK1(분시청수)적1.55、1.71배,시CK2(분시청수+접충백분균)적1.33、1.50배,차여CK1、CK2상비차이겁현저( P<0.01)。1.0 mmol/L SA유도(접균)후PAL、POD활성재제7(5)천시체도최대치,분별시CK1적1.68、2.06배,시CK2적1.35、1.56배,차여CK1、CK2상비차이겁현저( P<0.01)。0.5 mmol/L SA처리재정개시험기간유도적목질소상대치균현저고우CK1、CK2。
By sparying different concentration of salicylic acid (SA) on Rosa rugosa‘Plena’, we investigated their influence on the changes of several defense enzymes activity as well as secondary metabolite in leaves of R.rugosa‘Plena’.The effec-tiveness of different content SA on the resistance of Sphaerotheca pannosa (Wallr .) is obvious .SA spraying can signifi-cantly decrease disease indexes of S.pannosa (Wallr.) in R.rugosa‘Plena’ leaves especially by 0.5 mmol/L SA spra-ying .The activities of three kinds of protective enzymes , PAL, PPO and POD, the content of total phenols and the relative value of lignin are obviously induced by SA.After induced with 0.5 mmol/L SA, thePPO activities and the content of to-tal phenols reaches their maximum value on the seventh (fifth) day after inducing (inoculating of S.pannosa (Wallr.)), and their activities are 1.55 times (P<0.01) and 1.71 times (P<0.01) higher than that in control No.1 which is sprayed with pure water only (CK1), and were 1.33 times (P<0.01) and 1.50 times (P<0.01) higher than that in the control No.2 ( CK2) which is sprayed with pure water then inoculated with S.pannosa ( Wallr.) spores, and their those differ-ences are very significant.After induced with 1.0 mmol/L SA, the PAL and POD activities reach their maximum on the seventh (fifth) day after inducing (inoculating of S.pannosa (Wallr.)), and their activities are 1.68 times (P<0.01) and 2.06 times (P<0.01) higher than that of CK1, 1.35 times (P<0.01)and 1.56 times (P<0.01) higher than that of CK2, and those differences are obvious in significant level.After induced with 0.5 mmol/L SA, the relative value of lignin is significantly higher than that of CK1 and CK2 during the whole test.