CAJ | 학술논문

以肿节石斛（ Dendrobium pendulum）、尖刀唇石斛（ D．heterocarpum）和报春石斛（ D．primulinum）的无菌茎段为外植体，通过正交试验和单因子试验，研究不定芽诱导、生根和炼苗移栽等技术，筛选出各培养阶段的适宜培养基配方和培养条件，建立3种石斛兰离体快繁体系。结果表明：适合肿节石斛和报春石斛不定芽诱导的培养基为1/2MS＋6-BA 0．5 mg· L-1＋NAA 0．1 mg· L-1＋椰乳100 mL· L-1，诱导率分别为93．75％和90．00％；适合尖刀唇石斛不定芽诱导的培养基为1/2MS＋6-BA 0．25 mg· L-1＋NAA 0．1 mg· L-1＋椰乳100 mL· L-1，诱导率达到95．00％。适合肿节石斛和尖刀唇石斛生根的培养基为MS＋NAA 0．5 mg· L-1＋香蕉泥100 g· L-1，适合报春石斛生根的培养基为MS＋NAA 0．75 mg· L-1＋土豆泥100 g· L-1，生根诱导率高达100％。
이종절석곡（ Dendrobium pendulum）、첨도진석곡（ D．heterocarpum）화보춘석곡（ D．primulinum）적무균경단위외식체，통과정교시험화단인자시험，연구불정아유도、생근화련묘이재등기술，사선출각배양계단적괄의배양기배방화배양조건，건립3충석곡란리체쾌번체계。결과표명：괄합종절석곡화보춘석곡불정아유도적배양기위1/2MS＋6-BA 0．5 mg· L-1＋NAA 0．1 mg· L-1＋야유100 mL· L-1，유도솔분별위93．75％화90．00％；괄합첨도진석곡불정아유도적배양기위1/2MS＋6-BA 0．25 mg· L-1＋NAA 0．1 mg· L-1＋야유100 mL· L-1，유도솔체도95．00％。괄합종절석곡화첨도진석곡생근적배양기위MS＋NAA 0．5 mg· L-1＋향초니100 g· L-1，괄합보춘석곡생근적배양기위MS＋NAA 0．75 mg· L-1＋토두니100 g· L-1，생근유도솔고체100％。
An in vitro propagation system of Dendrobium pendulum, D.heterocarpum and D.primulinum was established with sterile stems as explants by single factor and orthogonal experimental design.Shoot multiplication and rooting were investi-gated to screen perfect mediums and culture conditions .The best shoot multiplication medium of D.pendulum and D. primulinum is 1/2MS medium supplemented with 0.5 mg· L-1 6-BA, 0.1 mg· L-1 NAA and 100 ml· L-1 CW, and the inducing rates are 93.75%and 90.00%, respectively.The best shoot multiplication medium of D.heterocarpum is 1/2MS me-dium supplemented with 0.25 mg· L-1 6-BA, 0.1 mg· L-1 NAA and 100 ml· L-1 CW, and the inducing rates were 95.00%. The best rooting medium of D.pendulum and D.heterocarpum is MS medium supplemented with 0.5 mg· L-1 NAA and 100 g· L-1 Banana Juice.The best rooting medium of D.primulinum is MS medium supplemented with 0.75 mg· L-1 NAA and 100 g· L-1 mashed potato with the rooting rate of 100%.