东北林业大学学报
東北林業大學學報
동북임업대학학보
JOURNAL OF NORTHEAST FORESTRY UNIVERSITY
2013年
8期
9-12,26
,共5页
脂松%自然越冬%低温胁迫%生理机理%越冬伤害
脂鬆%自然越鼕%低溫脅迫%生理機理%越鼕傷害
지송%자연월동%저온협박%생리궤리%월동상해
Red pine ( Pinus resinosa Ait.)%Natural overwintering%Low temperature stress%Physiological mecha-nism%Winter injury
研究不同处理（秋季涝渍、秋季变温、冬季遮荫）对2年生盆栽脂松苗木在自然越冬过程中针叶的膜脂过氧化作用、抗氧化酶活性和有机渗透调节物质的动态变化，并结合越冬伤害调查分析不同处理对其越冬伤害的影响。结果表明：在自然越冬期间各处理脂松苗木针叶的电导率、丙二醛（ MDA）质量摩尔浓度、脯氨酸质量分数及过氧化物酶（ POD）活性均表现为先升高后降低的变化趋势，可溶性糖质量分数表现为先升高后下降再升高，超氧化物歧化酶（SOD）活性在整个越冬期间总体的变化呈“M”形状；说明脂松苗木能够快速应对环境变化，通过调整生理代谢使叶片中快速积累渗透调节物和激活抗氧化酶防止氧自由基积累抑制膜脂过氧化，提高了脂松苗木组织内部的抗氧化能力、增强抗寒能力，从而在冬季低温变化过程中起到保护作用；各处理苗木均有不同程度的越冬伤害现象发生，冬季遮荫、秋季涝渍和秋季变温处理在3月份分别有10％、44％和100％的苗木发生越冬伤害现象，5月份的调查中分别有2％、10％和16％的苗木死亡。综合分析可见，脂松苗木具有较强的抗寒性，秋季骤然变温或秋季涝渍均会导致苗木在自然越冬过程中发生越冬伤害，而冬季适当遮荫有利于脂松安全越冬。
研究不同處理（鞦季澇漬、鞦季變溫、鼕季遮蔭）對2年生盆栽脂鬆苗木在自然越鼕過程中針葉的膜脂過氧化作用、抗氧化酶活性和有機滲透調節物質的動態變化，併結閤越鼕傷害調查分析不同處理對其越鼕傷害的影響。結果錶明：在自然越鼕期間各處理脂鬆苗木針葉的電導率、丙二醛（ MDA）質量摩爾濃度、脯氨痠質量分數及過氧化物酶（ POD）活性均錶現為先升高後降低的變化趨勢，可溶性糖質量分數錶現為先升高後下降再升高，超氧化物歧化酶（SOD）活性在整箇越鼕期間總體的變化呈“M”形狀；說明脂鬆苗木能夠快速應對環境變化，通過調整生理代謝使葉片中快速積纍滲透調節物和激活抗氧化酶防止氧自由基積纍抑製膜脂過氧化，提高瞭脂鬆苗木組織內部的抗氧化能力、增彊抗寒能力，從而在鼕季低溫變化過程中起到保護作用；各處理苗木均有不同程度的越鼕傷害現象髮生，鼕季遮蔭、鞦季澇漬和鞦季變溫處理在3月份分彆有10％、44％和100％的苗木髮生越鼕傷害現象，5月份的調查中分彆有2％、10％和16％的苗木死亡。綜閤分析可見，脂鬆苗木具有較彊的抗寒性，鞦季驟然變溫或鞦季澇漬均會導緻苗木在自然越鼕過程中髮生越鼕傷害，而鼕季適噹遮蔭有利于脂鬆安全越鼕。
연구불동처리（추계로지、추계변온、동계차음）대2년생분재지송묘목재자연월동과정중침협적막지과양화작용、항양화매활성화유궤삼투조절물질적동태변화，병결합월동상해조사분석불동처리대기월동상해적영향。결과표명：재자연월동기간각처리지송묘목침협적전도솔、병이철（ MDA）질량마이농도、포안산질량분수급과양화물매（ POD）활성균표현위선승고후강저적변화추세，가용성당질량분수표현위선승고후하강재승고，초양화물기화매（SOD）활성재정개월동기간총체적변화정“M”형상；설명지송묘목능구쾌속응대배경변화，통과조정생리대사사협편중쾌속적루삼투조절물화격활항양화매방지양자유기적루억제막지과양화，제고료지송묘목조직내부적항양화능력、증강항한능력，종이재동계저온변화과정중기도보호작용；각처리묘목균유불동정도적월동상해현상발생，동계차음、추계로지화추계변온처리재3월빈분별유10％、44％화100％적묘목발생월동상해현상，5월빈적조사중분별유2％、10％화16％적묘목사망。종합분석가견，지송묘목구유교강적항한성，추계취연변온혹추계로지균회도치묘목재자연월동과정중발생월동상해，이동계괄당차음유리우지송안전월동。
We studied the dynamic variations of membrane lipid peroxidation , antioxidant enzyme activity and organic osmoregu-lation substance in needles for two-year-old pot cultivation red pine seedlings under different treatments ( autumn waterlog-ging, autumn temperature fluctuation and winter shading) during natural overwintering.We investigated and analyzed the effects of different treatments on the winter injury.During natural overwintering, in red pine seedling needles under each treatment , the conductivity , the content of MDA, the content of proline and the activity of POD display the variation trend of increasing first and then declining, the content of solublesugar shows increase-decline-increase, the activity of SOD re-veals the overall variation shape of“M” during the whole overwintering .The red pine seedlings are able to rapidly respond to environment change.Osmoregulation substances accumulate rapidly in lead blades by regulating physiological metabo-lism and the accumulation of oxygen free radicals are prevented from inhibiting membrane lipid peroxidation by activating antioxidant enzyme to increase the internal antioxidant ability of red pine seedlings and strengthen the ability to resist cold with the protective effect in winter low temperature change .The winter injury phenomenon can occur in seedlings under each treatment.In March, under winter shading, autumn waterlogging and autumn temperature fluctuation, the winter in-jury occurs in 10%, 44%and 100%of seedlings, respectively.In May, there are 2%, 10%and 16%of died seedlings, respectively.Therefore, the seedling needles have stronger cold resistance, and abrupt temperature fluctuation in autumn or autumn waterlogging will lead to winter injury in seedlings during natural overwintering.However, appropriate shading in winter is favourable for safe overwintering of red pine.
